Transgenic Wheat, Barley and Oats

Production and Characterization Protocols

Editors:

ISBN: 978-1-58829-961-1 (Print) 978-1-59745-379-0 (Online)

Table of contents (20 protocols)

  1. Front Matter

    Pages i-x

  2. Introduction

    1. Front Matter

      Pages 1-1

    2. No Access

      Book Protocol

      Pages 3-20

      Transgenic Wheat, Barley and Oats: Production and Characterization

  3. Transformation and Regeneration

    1. Front Matter

      Pages 22-22

    2. No Access

      Book Protocol

      Pages 23-37

      Selection of Transformed Plants

    3. No Access

      Book Protocol

      Pages 39-69

      Reporter Genes

    4. No Access

      Book Protocol

      Pages 71-92

      Biolistics Transformation of Wheat

    5. No Access

      Book Protocol

      Pages 93-103

      Agrobacterium-Mediated Transformation of Bread and Durum Wheat Using Freshly Isolated Immature Embryos

    6. No Access

      Book Protocol

      Pages 105-113

      Floral Transformation of Wheat

    7. No Access

      Book Protocol

      Pages 115-124

      Highly Efficient Agrobacterium-Mediated Transformation of Wheat Via In Planta Inoculation

    8. No Access

      Book Protocol

      Pages 125-136

      Barley Transformation Using Biolistic Techniques

    9. No Access

      Book Protocol

      Pages 137-147

      Barley Transformation Using Agrobacterium-Mediated Techniques

    10. No Access

      Book Protocol

      Pages 149-168

      Transformation of Oats and Its Application to Improving Osmotic Stress Tolerance

  4. Gene and Protein Expression

    1. Front Matter

      Pages 170-170

    2. No Access

      Book Protocol

      Pages 171-184

      Promoter Sequences for Defining Transgene Expression

    3. No Access

      Book Protocol

      Pages 185-199

      Down-Regulation of Gene Expression by RNA-Induced Gene Silencing

  5. Characterisation of Transgenic Plants

    1. Front Matter

      Pages 202-202

    2. No Access

      Book Protocol

      Pages 203-226

      Gene Insertion Patterns and Sites

    3. No Access

      Book Protocol

      Pages 227-246

      Fluorescent In Situ Hybridization to Detect Transgene Integration into Plant Genomes

    4. No Access

      Book Protocol

      Pages 247-272

      Establishing Substantial Equivalence: Transcriptomics

    5. No Access

      Book Protocol

      Pages 273-288

      Establishing Substantial Equivalence: Proteomics

    6. No Access

      Book Protocol

      Pages 289-303

      Establishing Substantial Equivalence: Metabolomics

    7. No Access

      Book Protocol

      Pages 305-314

      Design and Management of Field Trials of Transgenic Cereals

    8. No Access

      Book Protocol

      Pages 315-330

      GM Risk Assessment

  6. Conclusions

    1. Front Matter

      Pages 332-332

    2. No Access

      Book Protocol

      Pages 333-345

      Transgenic Wheat, Barley and Oats: Future Prospects

  7. Back Matter

    Pages 347-349