Allergy Methods and Protocols

Editors:

ISBN: 978-0-89603-896-7 (Print) 978-1-59745-366-0 (Online)

Table of contents (27 protocols)

previous Page of 2
  1. Front Matter

    Pages i-xi

  2. No Access

    Book Protocol

    Pages 1-15

    Understanding of the Molecular Mechanisms of Allergy

  3. No Access

    Book Protocol

    Pages 17-30

    T Cell — Primary Culture from Peripheral Blood

  4. No Access

    Book Protocol

    Pages 31-41

    Production of T-Cell Lines

  5. No Access

    Book Protocol

    Pages 43-50

    Production of Human T-Cell Clones

  6. No Access

    Book Protocol

    Pages 51-56

    Mapping of Human T-Cell Epitopes of Allergens

  7. No Access

    Book Protocol

    Pages 57-72

    Determining MHC Restriction of T-cell Responses

  8. No Access

    Book Protocol

    Pages 73-83

    Short-Term Culture of CD8 Cells and Intracellular Cytokine Staining

  9. No Access

    Book Protocol

    Pages 85-96

    Isolation, Flow Cytometric Analysis, and Suppression Assay of CD4+ CD25+ T-Regulatory Cells

  10. No Access

    Book Protocol

    Pages 97-106

    Monocyte-Derived Dendritic Cells as Antigen-Presenting Cells in T-Cell Proliferation and Cytokine Production

  11. No Access

    Book Protocol

    Pages 107-119

    Ultrasensitive ELISA for Measurement of Human Cytokine Responses in Primary Culture

  12. No Access

    Book Protocol

    Pages 121-131

    Quantification of Human Chemokine Production in TLR-Stimulated and Antigen-Specific Recall Responses

  13. No Access

    Book Protocol

    Pages 133-145

    Standardization of Allergen Extracts

  14. No Access

    Book Protocol

    Pages 147-165

    Immunoelectrophoresis for the Characterization of Allergen Extracts

  15. No Access

    Book Protocol

    Pages 167-182

    Conjugation of Haptens

  16. No Access

    Book Protocol

    Pages 183-196

    Monoclonal Antibodies

  17. No Access

    Book Protocol

    Pages 197-207

    Purification of Antibodies

  18. No Access

    Book Protocol

    Pages 209-215

    Collection of Air Samples to Quantify Exposure to Airborne Allergens

  19. No Access

    Book Protocol

    Pages 217-225

    Assay of Air Sample Eluates

  20. No Access

    Book Protocol

    Pages 227-246

    The Halogen Assay - A New Technique for Measuring Airborne Allergen

  21. No Access

    Book Protocol

    Pages 247-254

    Measurement of Specific IgG Anti-Fel d 1 Antibodies

previous Page of 2