Adhesion Protein Protocols

Editors:

ISBN: 978-1-58829-533-0 (Print) 978-1-59745-353-0 (Online)

Table of contents (15 protocols)

  1. Front Matter

    Pages i-x

  2. No Access

    Book Protocol

    Pages 1-9

    Manipulation of Cell-Cell Adhesion Using Bowtie-Shaped Microwells

  3. No Access

    Book Protocol

    Pages 11-22

    Separation of Cell-Cell Adhesion Complexes by Differential Centrifugation

  4. No Access

    Book Protocol

    Pages 23-35

    Analysis of Neutrophil Chemotaxis

  5. No Access

    Book Protocol

    Pages 37-54

    Analysis of Leukocyte Migration Through Monolayers of Cultured Endothelial Cells

  6. No Access

    Book Protocol

    Pages 55-66

    Biochemical Purification of Pseudopodia from Migratory Cells

  7. No Access

    Book Protocol

    Pages 67-81

    Dynamic Assessment of Cell-Matrix Mechanical Interactions in Three-Dimensional Culture

  8. No Access

    Book Protocol

    Pages 83-95

    Quantitative Analyses of Cell Adhesion Strength

  9. No Access

    Book Protocol

    Pages 97-108

    Using RNA Interference to Knock Down the Adhesion Protein TES

  10. No Access

    Book Protocol

    Pages 109-119

    Assaying Calpain Activity

  11. No Access

    Book Protocol

    Pages 121-134

    Analysis of Focal Adhesions and Cytoskeleton by Custom Microarray

  12. No Access

    Book Protocol

    Pages 135-146

    Proteomic Analysis of Cell Surface Membrane Proteins in Leukemic Cells

  13. No Access

    Book Protocol

    Pages 147-171

    Bioinformatic Analysis of Adhesion Proteins

  14. No Access

    Book Protocol

    Pages 173-201

    Analysis of Integrin Dynamics by Fluorescence Recovery After Photobleaching

  15. No Access

    Book Protocol

    Pages 203-211

    Double-Hydrogel Substrate as a Model System for Three-Dimensional Cell Culture

  16. No Access

    Book Protocol

    Pages 213-226

    In Vitro Actin Assembly Assays and Purification From Acanthamoeba

  17. Back Matter

    Pages 227-234