Microarrays

Volume 1: Synthesis Methods

Editors:

ISBN: 978-1-58829-589-7 (Print) 978-1-59745-303-5 (Online)

Table of contents (22 protocols)

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  1. Front Matter

    Pages i-xix

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    Book Protocol

    Pages 1-36

    Introduction

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    Book Protocol

    Pages 37-57

    Current Microarray Surface Chemistries

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    Book Protocol

    Pages 59-92

    Nonfouling Surfaces

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    Book Protocol

    Pages 93-103

    Optimization of Oligonucleotide DNA Microarrays

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    Book Protocol

    Pages 105-119

    Detection of DNA Copy Number Alterations in Complex Genomes Using Array Comparative Genomic Hybridization

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    Book Protocol

    Pages 121-131

    Evaluating the Quality of Data From Microarray Measurements

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    Book Protocol

    Pages 133-163

    Construction of Oligonucleotide Microarrays (Biochip) Using Heterobifunctional Reagents

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    Book Protocol

    Pages 165-187

    Choice of Polymer Matrix, Its Functionalization and Estimation of Functional Group Density for Preparation of Biochips

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    Book Protocol

    Pages 189-211

    Methods in High-Resolution, Array-Based Comparative Genomic Hybridization

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    Book Protocol

    Pages 213-225

    Design and Fabrication of Spotted Long Oligonucleotide Microarrays for Gene Expression Analysis

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    Book Protocol

    Pages 227-246

    Construction of In Situ Oligonucleotide Arrays on Plastic

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    Book Protocol

    Pages 247-265

    Detecting Ligated Fragments on Oligonucleotide Microarrays

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    Book Protocol

    Pages 267-278

    Detection of Single-Nucleotide Polymorphisms in Cancer-Related Genes by Minisequencing on a Microelectronic DNA Chip

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    Book Protocol

    Pages 279-298

    Hybridization Analysis Using Oligonucleotide Probe Arrays

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    Book Protocol

    Pages 299-312

    In Situ Synthesis of Peptide Microarrays Using Ink-Jet Microdispensing

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    Book Protocol

    Pages 313-338

    Intein-Mediated Peptide Arrays for Epitope Mapping and Kinase/Phosphatase Assays

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    Book Protocol

    Pages 339-361

    Printing Low Density Protein Arrays in Microplates

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    Book Protocol

    Pages 363-373

    Forward-Phase and Reverse-Phase Protein Microarray

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    Book Protocol

    Pages 375-384

    Cell Microarray for Functional Exploration of Genomes

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    Book Protocol

    Pages 385-400

    Quantification of Mixed-Phase Hybridization on Polymer Microparticles by Europium (III) Ion Fluorescence

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