Adenovirus Methods and Protocols

Volume 2: Ad Proteins, RNA Lifecycle, Host Interactions, and Phylogenetics

Editors:

ISBN: 978-1-58829-901-7 (Print) 978-1-59745-277-9 (Online)

Table of contents (23 protocols)

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  1. Front Matter

    Pages i-xiii

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    Pages 1-14

    Analysis of the Efficiency of Adenovirus Transcription

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    Pages 15-31

    The Use of In Vitro Transcription to Probe Regulatory Functions of Viral Protein Domains

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    Pages 33-46

    Preparation of Soluble Extracts From Adenovirus-Infected Cells for Studies of RNA Splicing

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    Pages 47-61

    In Vitro Methods to Study RNA Interference During an Adenovirus Infection

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    Pages 63-72

    Simultaneous Detection of Adenovirus RNA and Cellular Proteins by Fluorescent Labeling In Situ

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    Pages 73-81

    Study of Nucleolar Localization of Adenovirus Core Proteins

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    Pages 83-101

    Analysis of Adenovirus Infections in Synchronized Cells

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    Pages 103-111

    Co-Immunoprecipitation of Protein Complexes

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    Pages 113-121

    Chromatin Immunoprecipitation to Study the Binding of Proteins to the Adenovirus Genome In Vivo

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    Pages 123-139

    Assaying Protein-DNA Interactions In Vivo and In Vitro Using Chromatin Immunoprecipitation and Electrophoretic Mobility Shift Assays

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    Pages 141-155

    Identifying Functional Adenovirus-Host Interactions Using Tandem Mass Spectrometry

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    Pages 157-185

    The Use of Cell Microinjection for the In Vivo Analysis of Viral Transcriptional Regulatory Protein Domains

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    Pages 187-195

    Determination of the Transforming Activities of Adenovirus Oncogenes

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    Pages 197-211

    Human Adenovirus Type 12

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    Pages 213-219

    Measurement of Natural-Killer Cell Lytic Activity of Adenovirus-Infected or Adenovirus-Transformed Cells

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    Pages 221-230

    A Flow Cytometric Assay for Analysis of Natural-Killer Cell-Mediated Cytolysis of Adenovirus-Transformed Cells

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    Pages 231-250

    Large-Scale Purification and Crystallization of Adenovirus Hexon

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    Pages 251-255

    Synthesis and Assay of Recombinant Adenovirus Protease

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    Pages 257-267

    Assay for the Adenovirus Proteinase

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    Pages 269-280

    Cofactors of the Adenovirus Proteinase

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