New Antibiotic Targets

Editors:

ISBN: 978-1-58829-915-4 (Print) 978-1-59745-246-5 (Online)

Table of contents (20 protocols)

  1. Front Matter

    Pages i-xi

  2. No Access

    Book Protocol

    Pages 1-9

    Biocomputational Strategies for Microbial Drug Target Identification

  3. No Access

    Book Protocol

    Pages 11-23

    Methods to Assay Inhibitors of DNA Gyrase and Topoisomerase IV Activities

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    Book Protocol

    Pages 25-36

    A Method to Assay Inhibitors of DNA Polymerase IIIC Activity

  5. No Access

    Book Protocol

    Pages 37-51

    Methods to Identify and Characterize Inhibitors of Bacterial RNA Polymerase

  6. No Access

    Book Protocol

    Pages 53-61

    Methods to Assay Inhibitors of tRNA Synthetase Activity

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    Book Protocol

    Pages 63-73

    Three Methods to Assay Inhibitors of Ribosomal Subunit Assembly

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    Book Protocol

    Pages 75-85

    Inhibition ofChaperone-Dependent Bacterial Ribosome Biogenesis

  9. No Access

    Book Protocol

    Pages 87-106

    Assays for the Identification of Inhibitors Targeting Specific Translational Steps

  10. No Access

    Book Protocol

    Pages 107-116

    SPARK: A New Peptidyl Transferase Activity Assay

  11. No Access

    Book Protocol

    Pages 117-130

    High-Throughput Screening of Peptide Deformylase Inhibitors

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    Pages 131-141

    A Method to Assay Penicillin-Binding Proteins

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    Book Protocol

    Pages 143-154

    A Method to Assay Inhibitors of Lipopolysaccharide Synthesis

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    Book Protocol

    Pages 155-173

    Methods for Assessing the Structure and Function of Cationic Antimicrobial Peptides

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    Book Protocol

    Pages 175-186

    Flow Cytometry of Bacterial Membrane Potential and Permeability

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    Book Protocol

    Pages 187-204

    Bacterial Efflux Pump Inhibitors

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    Book Protocol

    Pages 205-213

    Mycobacterium tuberculosis β-Ketoacyl Acyl Carrier Protein Synthase III (mtFabH) Assay: Principles and Method

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    Book Protocol

    Pages 215-222

    Screening for Compounds That Affect the Interaction Between Bacterial Two-Component Signal Transduction Response Regulator Protein and Cognate Promoter DNA

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    Book Protocol

    Pages 223-237

    The Activity of rRNA Resistance Methyltransferases Assessed by MALDI Mass Spectrometry

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    Book Protocol

    Pages 239-260

    Assays for Β-Lactamase Activity and Inhibition

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    Book Protocol

    Pages 261-271

    Studies of Enzymes That Cause Resistance to Aminoglycosides Antibiotics

  22. Back Matter

    Pages 273-274