Adipose Tissue Protocols

Editors:

ISBN: 978-1-58829-916-1 (Print) 978-1-59745-245-8 (Online)

Table of contents (24 protocols)

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  1. Front Matter

    Pages i-xv

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    Pages 1-22

    Overview of Adipose Tissue and Its Role in Obesity and Metabolic Disorders

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    Pages 23-38

    Choosing an Adipose Tissue Depot for Sampling

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    Pages 39-54

    Application of Imaging and Other Noninvasive Techniques in Determining Adipose Tissue Mass

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    Pages 55-64

    Generation of Adipose Tissue-Specific Transgenic Mouse Models

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    Pages 65-81

    Angiogenesis in Adipose Tissue

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    Pages 83-95

    Adipose Organ Nerves Revealed by Immunohistochemistry#

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    Pages 97-107

    Human Adipose Tissue Blood Flow and Micromanipulation of Human Subcutaneous Blood Flow

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    Pages 109-121

    Studies of Thermogenesis and Mitochondrial Function in Adipose Tissues

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    Pages 123-130

    Application of Lipidomics and Metabolomics to the Study of Adipose Tissue

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    Pages 131-140

    Applications of Proteomics to the Study of Adipose Tissue

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    Pages 141-154

    Application of DNA Microarray to the Study of Human Adipose Tissue/Cells

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    Pages 155-171

    Differentiation of Adipose Stem Cells

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    Pages 173-193

    Methods That Resolve Different Contributions of Clonal Expansion to Adipogenesis in 3T3-L1 and C3H10T1/2 Cells

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    Pages 195-199

    Explant Cultures of White Adipose Tissue

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    Pages 201-219

    Isolation and Culture of Preadipocytes from Rodent White Adipose Tissue

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    Pages 221-229

    Flow Cytometry on the Stromal-Vascular Fraction of White Adipose Tissue

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    Pages 231-247

    Application of Electrophoretic Mobility Shift Assay and Chromatin Immunoprecipitation in the Study of Transcription in Adipose Cells

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    Pages 249-257

    Application of RNA Interference Techniques to Adipose Cell Cultures

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    Pages 259-270

    RNA Isolation and Real-Time Quantitative RT-PCR

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    Pages 271-283

    Study of Adipose Tissue Gene Expression by In Situ Hybridization

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