Book Volume 358 2007

Metabolomics

Methods and Protocols

Editors:

ISBN: 978-1-58829-561-3 (Print) 978-1-59745-244-1 (Online)

Table of contents (15 protocols)

  1. Front Matter

    Pages i-xii

  2. Metabolome Analysis Using Gas Chromatography Coupled to Mass Spectrometry

    1. Front Matter

      Pages 1-1

    2. No Access

      Protocol

      Pages 3-17

      Metabolite Profiling in Blood Plasma

    3. No Access

      Protocol

      Pages 19-38

      Nonsupervised Construction and Application of Mass Spectral and Retention Time Index Libraries From Time-of-Flight Gas Chromatography-Mass Spectrometry Metabolite Profiles

    4. No Access

      Protocol

      Pages 39-53

      Metabolomic Profiling of Natural Volatiles

  3. Metabolomics: Data Integration and Data Mining

    1. Front Matter

      Pages 17-17

    2. No Access

      Protocol

      Pages 57-75

      Integrative Profiling of Metabolites and Proteins

    3. No Access

      Protocol

      Pages 77-85

      Integrating Profiling Data

    4. No Access

      Protocol

      Pages 87-104

      Visualization and Analysis of Molecular Data

    5. No Access

      Protocol

      Pages 105-126

      A Gentle Guide to the Analysis of Metabolomic Data

  4. Capillary Electrophoresis Coupled to Mass Spectrometry

    1. Front Matter

      Pages 37-37

    2. No Access

      Protocol

      Pages 129-137

      Capillary Electrophoresis-Mass Spectrometry for Metabolomics

  5. Liquid Chromatography Coupled to Mass Spectrometry for Metabolomics and Structural Elucidation

    1. Front Matter

      Pages 55-55

    2. No Access

      Protocol

      Pages 141-155

      Application of Liquid Chromatography-Mass Spectrometry Analysis in Metabolomics

  6. Electrochemical Detection of Metabolites

    1. Front Matter

      Pages 75-75

    2. No Access

      Protocol

      Pages 159-174

      High-Performance Liquid Chromatography Separations Coupled With Coulometric Electrode Array Detectors

  7. Metabolic Fluxes

    1. Front Matter

      Pages 175-175

    2. No Access

      Protocol

      Pages 177-197

      Determination of Metabolic Flux Ratios From 13C-Experiments and Gas Chromatography-Mass Spectrometry Data

    3. No Access

      Protocol

      Pages 199-226

      Understanding the Roadmap of Metabolism by Pathway Analysis

  8. NMR Metabolome Analysis

    1. Front Matter

      Pages 103-103

    2. No Access

      Protocol

      Pages 229-246

      Revealing the Metabolome of Animal Tissues Using 1H Nuclear Magnetic Resonance Spectroscopy

    3. No Access

      Protocol

      Pages 247-271

      Nuclear Magnetic Resonance Metabonomics

    4. No Access

      Protocol

      Pages 273-286

      Practical Aspects of Uniform Stable Isotope Labeling of Higher Plants for Heteronuclear NMR-Based Metabolomics

  9. Back Matter

    Pages 287-293