Gene Therapy Protocols

Production and In Vivo Applications of Gene Transfer Vectors

Authors:

ISBN: 978-1-58829-903-1 (Print) 978-1-59745-237-3 (Online)

Table of contents (22 protocols)

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  1. Front Matter

    Pages i-xii

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    Pages 1-16

    Preparation and Quantification of Pseudotyped Retroviral Vector

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    Pages 17-32

    Production of Retroviral Vectors for Clinical Use

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    Pages 33-54

    Methods for the Production of Helper-Dependent Adenoviral Vectors

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    Pages 55-78

    Methods for the Production of First Generation Adenoviral Vectors

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    Pages 79-96

    Large-Scale Production of Recombinant Adeno-Associated Viral Vectors

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    Pages 97-103

    Construction and Production of Recombinant Herpes Simplex Virus Vectors

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    Pages 115-125

    Plasmid-Based Gene Transfer in Mouse Skeletal Muscle by Electroporation

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    Pages 127-140

    Chitosan Nanoparticle-Mediated Gene Transfer

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    Pages 141-150

    PEG–PEI Copolymers for Oligonucleotide Delivery to Cells and Tissues

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    Pages 159-175

    Non-Viral Gene Delivery with Cationic Liposome–DNA Complexes

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    Pages 177-202

    Applications of Lentiviral Vectors in Noninvasive Molecular Imaging

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    Pages 203-212

    Retroviral Modification of Mesenchymal Stem Cells for Gene Therapy of Hemophilia

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    Pages 213-228

    Transduction of Murine Hematopoietic Stem Cells and In Vivo Selection of Gene-Modified Cells

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    Pages 229-242

    Assessment of CFTR Function after Gene Transfer In Vitro and In Vivo

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    Pages 243-258

    Oncolytic Adenoviruses for Cancer Gene Therapy

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    Pages 259-276

    Design of Trans-Splicing Adeno-Associated Viral Vectors for Duchenne Muscular Dystrophy Gene Therapy

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    Pages 277-286

    Prevention of Type 1 Diabetes in NOD Mice by Genetic Engineering of Hematopoietic Stem Cells

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    Pages 287-300

    Lentiviral Vector Delivery of siRNA and shRNA Encoding Genes into Cultured and Primary Hematopoietic Cells

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    Pages 301-332

    Nanoparticle-Mediated Gene Delivery to the Lung

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    Pages 333-354

    Retroviral-Mediated Gene Therapy for the Differentiation of Primary Cells into a Mineralizing Osteoblastic Phenotype

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