Prion Protein Protocols

Editors:

ISBN: 978-1-58829-897-3 (Print) 978-1-59745-234-2 (Online)

Table of contents (18 protocols)

  1. Front Matter

    Pages i-xiii

  2. No Access

    Book Protocol

    Pages 1-20

    Cell Culture Models to Unravel Prion Protein Function and Aberrancies in Prion Diseases

  3. No Access

    Book Protocol

    Pages 21-34

    Investigation of PrPC Metabolism and Function in Live Cells

  4. No Access

    Book Protocol

    Pages 35-48

    Immunodetection of PrPSc Using Western and Slot Blotting Techniques

  5. No Access

    Book Protocol

    Pages 49-68

    Assaying Prions in Cell Culture

  6. No Access

    Book Protocol

    Pages 69-82

    Generation of Cell Lines Propagating Infectious Prions and the Isolation and Characterization of Cell-derived Exosomes

  7. No Access

    Book Protocol

    Pages 83-96

    Neurotoxicity of Prion Peptides on Cultured Cerebellar Neurons

  8. No Access

    Book Protocol

    Pages 97-103

    Understanding the Nature of Prion Diseases Using Cell-free Assays

  9. No Access

    Book Protocol

    Pages 105-115

    Methods for Conversion of Prion Protein into Amyloid Fibrils

  10. No Access

    Book Protocol

    Pages 117-130

    Amplification of Purified Prions In Vitro

  11. No Access

    Book Protocol

    Pages 131-143

    Expression and Purification of Full-Length Recombinant PrP of High Purity

  12. No Access

    Book Protocol

    Pages 145-159

    Analysis of PrP Conformation Using Circular Dichroism

  13. No Access

    Book Protocol

    Pages 161-172

    Effect of Copper on the De Novo Generation of Prion Protein Expressed in Pichia pastoris

  14. No Access

    Book Protocol

    Pages 173-196

    Biophysical Investigations of the Prion Protein Using Electron Paramagnetic Resonance

  15. No Access

    Book Protocol

    Pages 197-227

    Molecular Diagnosis of Human Prion Disease

  16. No Access

    Book Protocol

    Pages 229-239

    Analysis of Endogenous PrPC Processing in Neuronal and Non-neuronal Cell Lines

  17. No Access

    Book Protocol

    Pages 241-247

    Molecular Typing of PrPres in Human Sporadic CJD Brain Tissue

  18. No Access

    Book Protocol

    Pages 249-263

    Transgenic Mouse Models of Prion Diseases

  19. No Access

    Book Protocol

    Pages 265-273

    Quantitative Bioassay of Surface-bound Prion Infectivity

  20. Back Matter

    Pages 275-280