Host-Pathogen Interactions

Methods and Protocols

Editors:

ISBN: 978-1-58829-886-7 (Print) 978-1-59745-204-5 (Online)

Table of contents (26 protocols)

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  1. Front Matter

    Pages i-xi

  2. Bacterial Pathogens

    1. Front Matter

      Pages 1-1

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      Pages 3-4

      Introduction: Bacterial Pathogens

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      Pages 5-27

      Transcriptome Analyses in the Interaction of Neisseria meningitidis with Mammalian Host Cells

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      Pages 29-45

      Surface-Exposed Adherence Molecules of Streptococcus pneumoniae

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      Pages 47-56

      Screening of Virulence Traits in Legionella pneumophila and Analysis of the Host Susceptibility to Infection by Using the Dictyostelium Host Model System

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      Pages 57-65

      Characterizing Host Receptor Recognition by Individual Bacterial Pathogens

  3. Fungal Pathogens

    1. Front Matter

      Pages 67-67

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      Pages 69-70

      Introduction: Fungal Pathogens

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      Pages 71-83

      Isolation and Purification of Antigenic Components of Cryptococcus

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      Pages 85-94

      A Method for Examining Glycans Surface Expression of Yeasts by Flow Cytometry

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      Pages 95-104

      Human Epithelial Model Systems for the Study of Candida Infections In Vitro: Part I. Adhesion to Epithelial Models

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      Pages 105-123

      Human Epithelial Model Systems for the Study of Candida infections In Vitro: Part II. Histologic Methods for Studying Fungal Invasion

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      Pages 125-139

      In Vitro Systems for Studying the Interaction of Fungal Pathogens with Primary Cells from the Mammalian Innate Immune System

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      Pages 141-149

      Experimental Infection of Rodent Mammals for Fungal Virulence Testing

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      Pages 151-167

      Real-Time and Semiquantitative RT-PCR Methods to Analyze Gene Expression Patterns During Aspergillus-Host Interactions

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      Pages 169-185

      Antibody-Based Strategy to Identify Candida albicans Genes Expressed During Infections

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      Pages 187-235

      Identification of the Candida albicans Immunome During Systemic Infection by Mass Spectrometry

  4. Parasites

    1. Front Matter

      Pages 237-237

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      Pages 239-243

      Introduction: Parasites

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      Pages 245-262

      Axenic In Vitro Cultivation of Echinococcus multilocularis Metacestode Vesicles and the Generationof Primary Cell Cultures

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      Pages 263-273

      Culture of Exoerythrocytic Stages of the Malaria Parasites Plasmodium falciparum and Plasmodium vivax

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      Pages 275-288

      Modulation of Caspase Activation by Toxoplasma gondii

  5. Host Responses

    1. Front Matter

      Pages 289-289

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      Pages 291-292

      Introduction: Host Responses

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