Microinjection

Methods and Applications

Editors:

ISBN: 978-1-58829-884-3 (Print) 978-1-59745-202-1 (Online)

Table of contents (15 protocols)

  1. Front Matter

    Pages i-xvi

  2. No Access

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    Pages 1-15

    Expression of Exogenous mRNA in Xenopus laevis Embryos for the Study of Cell Cycle Regulation

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    Pages 17-29

    Use of Luciferase Chimaera to Monitor PLCζ Expression in Mouse Eggs

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    Pages 31-41

    Analysis of 14-3-3 Family Member Function in Xenopus Embryos by Microinjection of Antisense Morpholino Oligos

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    Pages 43-55

    A Microinjectable Biological System, the Xenopus Oocyte, as an Approach to Understanding Signal Transduction Protein Function

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    Pages 57-66

    Combining Microinjection and Immunoblotting to Analyze MAP Kinase Phosphorylation in Single Starfish Oocytes and Eggs

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    Pages 67-76

    Analysis of Signaling Pathways in Zebrafish Development by Microinjection

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    Pages 77-97

    Protein Inhibition by Microinjection and RNA-Mediated Interference in Tissue Culture Cells: Complementary Approaches to Study Protein Function

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    Pages 99-112

    DNA Delivery by Microinjection for the Generation of Recombinant Mammalian Cell Lines

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    Pages 113-122

    Bacteriophage φC31 Integrase Mediated Transgenesis in Xenopus laevis for Protein Expression at Endogenous Levels

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    Pages 123-133

    Germline Transformation of Caenorhabditis elegans by Injection

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    Pages 135-156

    Quantitative Microinjection of Mouse Oocytes and Eggs

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    Pages 157-173

    Microinjection of Follicle-Enclosed Mouse Oocytes

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    Pages 175-188

    Functional Studies of Regulatory Genes in the Sea Urchin Embryo

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    Pages 189-206

    Exploring the Cytoskeleton During Intracytoplasmic Sperm Injection in Humans

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    Pages 207-218

    Somatic Cell Nuclear Transfer in the Mouse

  17. Back Matter

    Pages 219-221