The Protein Protocols Handbook

Editors:

ISBN: 978-1-60327-474-6 (Print) 978-1-59745-198-7 (Online)

Table of contents (208 protocols)

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  1. Front Matter

    Pages i-xxxv

  2. Quantitation of Proteins

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      Pages 3-6

      Protein Determination by UV Absorption

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      Pages 7-10

      The Lowry Method for Protein Quantitation

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      Pages 11-15

      The Bicinchoninic Acid (BCA) Assay for Protein Quantitation

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      Pages 17-24

      The Bradford Method For Protein Quantitation

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      Pages 25-34

      Ultrafast Protein Determinations Using Microwave Enhancement

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      Pages 35-45

      The Nitric Acid Method for Protein Estimation in Biological Samples

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      Pages 47-50

      Quantitation of Tryptophan in Proteins

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      Pages 51-54

      Kinetic Silver Staining of Proteins

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      Pages 55-61

      Quantitation of Cellular Proteins by Flow Cytometry

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      Pages 63-69

      Quantitation of Cellular Proteins by Laser Scanning Cytometry

  3. Electrophoresis of Proteins and Peptides and Dectection in Gels

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      Pages 73-84

      Protein Solubility in Two-Dimensional Electrophoresis

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      Pages 85-107

      Mouse and Human Tissue Sample Preparation for 2-D Electrophoresis

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      Pages 109-119

      Plant Protein Sample Preparation for 2-DE

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      Pages 121-130

      Preparation of Bacterial Samples for 2-D PAGE

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      Pages 131-138

      Preparation of Bodily Fluids for 2-D PAGE

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      Pages 139-153

      Immunoaffinity Depletion of High Abundance Plasma and Serum Proteins

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      Pages 155-158

      Preparation of Yeast Samples for 2-D PAGE

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      Pages 159-164

      Membrane Protein Preparation Using Aqueous Polymer Two-Phase Systems

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      Pages 165-170

      Subcellular Fractionation of Small Sample Amounts

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      Pages 171-176

      Nondenaturing Polyacrylamide Gel Electrophoresis of Proteins

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