Electroporation Protocols

Preclinical and Clinical Gene Medicine

Editors:

ISBN: 978-1-58829-877-5 (Print) 978-1-59745-194-9 (Online)

Table of contents (40 protocols)

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  1. Front Matter

    Pages i-xxi

  2. Basic Concepts of DNA Transfer via Electroporation

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      Pages 3-17

      Application of Electroporation Gene Therapy: Past, Current, and Future

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      Pages 19-33

      Mechanism by Which Electroporation Mediates DNA Migration and Entry into Cells and Targeted Tissues

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      Pages 35-59

      Applicator and Electrode Design for In Vivo DNA Delivery by Electroporation

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      Pages 61-76

      Electrode Assemblies Used for Electroporation of Cultured Cells

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      Pages 77-89

      Formulations for DNA Delivery via Electroporation In Vivo

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      Pages 91-107

      Overview of Drug Delivery and Alternative Methods to Electroporation

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      Pages 109-128

      Nanoelectroporation: A First Look

  3. In Vitro Targeted Gene Delivery via Electroporation

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      Pages 131-138

      Nucleofection of Human Embryonic Stem Cells

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      Pages 139-153

      Delivery of Whole Tumor Lysate into Dendritic Cells for Cancer Vaccination

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      Pages 155-163

      Delivery of Tumor-Antigen-Encoding mRNA into Dendritic Cells for Vaccination

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      Pages 165-172

      Delivery of DNA into Natural Killer Cells for Immunotherapy

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      Pages 173-189

      Electroporation of Adherent Cells In Situ for the Study of Signal Transduction and Gap Junctional Communication

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      Pages 191-195

      Delivery of DNA into Adipocytes within Adipose Tissue

  4. In Vivo Targeted Gene Delivery via Electroporation

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      Pages 199-214

      Delivery of DNA into Muscle for Treating Systemic Diseases: Advantages and Challenges

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      Pages 215-224

      Delivery of DNA into Skeletal Muscle in Large Animals

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      Pages 225-232

      Delivery of DNA into Skin via Electroporation

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      Pages 233-247

      Electroporation-Mediated Gene Delivery to the Lungs

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      Pages 249-257

      Delivery of DNA into Bladder via Electroporation

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      Pages 259-278

      Analysis of Gene Function in the Retina

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      Pages 279-287

      Optical In Vivo Imaging of Electrically Mediated Delivery of siRNA into Muscle for Gene Function Analysis

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