Genomics Protocols

Editors:

ISBN: 978-1-58829-871-3 (Print) 978-1-59745-188-8 (Online)

Table of contents (27 protocols)

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  1. Front Matter

    Pages i-xv

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    Pages 1-18

    Whole Genome Amplification with Phi29 DNA Polymerase to Enable Genetic or Genomic Analysis of Samples of Low DNA Yield

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    Pages 19-34

    Scanning for DNA Variants by Denaturant Capillary Electrophoresis

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    Pages 35-52

    Identification of SNPs, or Mutations in Sequence Chromatograms

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    Pages 53-74

    BeadArray-Based Genotyping

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    Pages 75-86

    Microsatellite-Based Candidate Gene Linkage Analysis Studies

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    Pages 87-100

    Full Complexity Genomic Hybridization on 60-mer Oligonucleotide Microarrays for Array Comparative Genomic Hybridization (aCGH)

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    Pages 101-108

    Detection of Copy Number Changes at Multiple Loci in DNA Prepared from Formalin-Fixed, Paraffin-Embedded Tissue by Multiplex Ligation-Dependent Probe Amplification

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    Pages 109-129

    Application of Microarrays for DNA Methylation Profiling

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    Pages 131-145

    Genomewide Identification of Protein Binding Locations Using Chromatin Immunoprecipitation Coupled with Microarray

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    Pages 147-158

    Transcriptional Profiling of Small Samples in the Central Nervous System

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    Pages 159-177

    Quantitative Expression Profiling of RNA from Formalin-Fixed, Paraffin-Embedded Tissues Using Randomly Assembled Bead Arrays

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    Pages 179-190

    Expression Profiling of microRNAs in Cancer Cells: Technical Considerations

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    Pages 191-197

    Identification of Disease Biomarkers by Profiling of Serum Proteins Using SELDI-TOF Mass Spectrometry

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    Pages 199-209

    The Applicability of a Cluster of Differentiation Monoclonal Antibody Microarray to the Diagnosis of Human Disease

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    Pages 211-224

    Protein Profiling Based on Two-Dimensional Difference Gel Electrophoresis

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    Pages 225-240

    Quantitative Protein Profiling by Mass Spectrometry Using Isotope-Coded Affinity Tags

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    Pages 241-256

    Quantitative Protein Profiling by Mass Spectrometry Using Label-Free Proteomics

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    Pages 257-267

    Using 2D-LC-MS/MS to Identify Francisella tularensis Peptides in Extracts from an Infected Mouse Macrophage Cell Line

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    Pages 269-289

    Baculovirus Expression Vector System: An Emerging Host for High-Throughput Eukaryotic Protein Expression

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    Pages 291-308

    Coimmunoprecipitation and Proteomic Analyses

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