Exocytosis and Endocytosis

Editors:

ISBN: 978-1-58829-865-2 (Print) 978-1-59745-178-9 (Online)

Table of contents (28 protocols)

previous Page of 2
  1. Front Matter

    Pages i-xv

  2. An Overview of Methods for Analyzing Vesicular Trafficking

    1. No Access

      Book Protocol

      Pages 3-14

      Vesicular Trafficking: Molecular Tools and Targets

    2. No Access

      Book Protocol

      Pages 15-33

      Pharmacological Inhibition of Endocytic Pathways: Is It Specific Enough to Be Useful?

  3. Cell-Free and Biochemical Assays of Exocytosis and Endocytosis

    1. No Access

      Book Protocol

      Pages 37-50

      In Vitro Assays to Measure SNARE-Mediated Vesicle Fusion

    2. No Access

      Book Protocol

      Pages 51-59

      Imaging Ca2+-Triggered Exocytosis of Single Secretory Granules on Plasma Membrane Lawns from Neuroendocrine Cells

    3. No Access

      Book Protocol

      Pages 61-76

      FRAP Analysis of Secretory Granule Lipids and Proteins in the Sea Urchin Egg

    4. No Access

      Book Protocol

      Pages 77-87

      A Cell-Free Assay for Endocytosis of E-Cadherin

    5. No Access

      Book Protocol

      Pages 89-96

      Cell-Surface Biotinylation to Study Endocytosis and Recycling of Occludin

    6. No Access

      Book Protocol

      Pages 97-110

      Fractionation of Subcellular Membrane Vesicles of Epithelial and Nonepithelial Cells by OptiPrep™ Density Gradient Ultracentrifugation

    7. No Access

      Book Protocol

      Pages 111-125

      Identification and Characterization of Interacting Partners of Rab GTPases by Yeast Two-Hybrid Analyses

  4. Analysis of Endocytosis and Exocytosis in Different Cell Systems

    1. No Access

      Book Protocol

      Pages 129-146

      High-Throughput Analysis of the Dynamics of Recycling Cell Surface Proteins

    2. No Access

      Book Protocol

      Pages 147-155

      Studying Phagocytosis by Live-Cell Scintillation Proximity Assay

    3. No Access

      Book Protocol

      Pages 157-170

      Transcytosis of Polymeric Immunoglobulin A in Polarized Madin–Darby Canine Kidney Cells

    4. No Access

      Book Protocol

      Pages 171-186

      The Use of Syntaxin Chimeras to Study Polarized Protein Trafficking in Epithelial Cells

    5. No Access

      Book Protocol

      Pages 187-201

      Targeting the Epithelial SNARE Machinery by Bacterial Neurotoxins

    6. No Access

      Book Protocol

      Pages 203-215

      Exocytosis of Endothelial Cells Is Regulated by N-Ethylmaleimide-Sensitive Factor

    7. No Access

      Book Protocol

      Pages 217-226

      Molecular Dissection of HCl Secretion in Gastric Parietal Cells Using Streptolysin O Permeabilization

    8. No Access

      Book Protocol

      Pages 227-233

      Addressing Membrane Protein Topology Using the Fluorescence Protease Protection (FPP) Assay

    9. No Access

      Book Protocol

      Pages 235-245

      Visualizing Clathrin-Mediated IgE Receptor Internalization by Electron and Atomic Force Microscopy

    10. No Access

      Book Protocol

      Pages 247-257

      Analyzing Endosomes in Nonsectioned Cells by Transmission Electron Microscopy

    11. No Access

      Book Protocol

      Pages 259-268

      Imaging Exocytosis of Single Insulin Secretory Granules With TIRF Microscopy

previous Page of 2