HIV Protocols

Editors:

ISBN: 978-1-58829-859-1 (Print) 978-1-59745-170-3 (Online)

Table of contents (30 protocols)

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  1. Front Matter

    Pages i-xiv

  2. Preparation of Virus Particles and their Analysis

    1. Front Matter

      Pages 1-1

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      Pages 3-14

      Methods for Viral RNA Isolation and PCR Amplification for Sequencing of Near Full-Length HIV-1 Genomes

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      Pages 15-25

      Purification of HIV-1 Virions by Subtilisin Digestion or CD45 Immunoaffinity Depletion for Biochemical Studies

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      Pages 27-35

      Calculating HIV-1 Infectious Titre Using a Virtual TCID50 Method

  3. Methods To Study Hiv-1 Replication

    1. Front Matter

      Pages 36-37

    2. Early Events

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        Pages 41-53

        Cell-Free Assays for HIV-1 Uncoating

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        Pages 55-72

        Real-Time PCR Analysis of HIV-1 Replication Post-entry Events

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        Pages 73-85

        Analysis of 2-LTR Circle Junctions of Viral DNA in Infected Cells

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        Pages 87-105

        HIV-1 Recombination: An Experimental Assay and a Phylogenetic Approach

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        Pages 107-119

        Methods of Preparation and Analysis of Intracellular Reverse Transcription Complexes

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        Pages 121-134

        Analysis of Viral and Cellular Proteins in HIV-1 Reverse Transcription Complexes by Co-immunoprecipitation

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        Pages 135-149

        Isolation and Analysis of HIV-1 Preintegration Complexes

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        Pages 151-159

        Bisarsenical Labeling of HIV-1 for Real-Time Fluorescence Microscopy

      Late Events

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        Pages 163-184

        Methods for the Study of HIV-1 Assembly

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        Pages 185-195

        Assembly of Immature HIV-1 Capsids Using a Cell-Free System

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        Pages 197-208

        Preparation of Recombinant HIV-1 Gag Protein and Assembly of Virus-Like Particles In Vitro

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        Pages 209-221

        Methods for the Analysis of HIV-1 Nucleocapsid Protein Interactions with Oligonucleotides

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        Pages 223-232

        Methods for Analysis of Incorporation and Annealing Methods for Analysis of tRNALys in HIV-1

  4. Specialized Approaches To Study Hiv-1 Biology and Pathogenesis

    1. Front Matter

      Pages 233-233

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      Pages 235-255

      Somatic Cell Genetic Analyses to Identify HIV-1 Host Restriction Factors

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      Pages 257-270

      Rapid, Controlled and Intensive Lentiviral Vector-Based RNAi

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      Pages 271-293

      Reverse Two-Hybrid Screening to Analyze Protein–Protein Interaction of HIV-1 Viral and Cellular Proteins

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      Pages 295-309

      Methods to Study Monocyte Migration Induced by HIV-Infected Cells

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