Plant Virology Protocols

From Viral Sequence to Protein Function

ISBN: 978-1-58829-827-0 (Print) 978-1-59745-102-4 (Online)

Table of contents (44 protocols)

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  1. Front Matter

    Pages i-xx

  2. General Introduction

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      Pages 3-19

      Plant–Virus Interactions

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      Pages 21-31

      Role of Capsid Proteins

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      Pages 33-54

      Role of Plant Virus Movement Proteins

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      Pages 55-68

      Multiple Roles of Viral Replication Proteins in Plant RNA Virus Replication

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      Pages 69-79

      Role of Silencing Suppressor Proteins

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      Pages 81-96

      Role of Vector-Transmission Proteins

  3. Viral Nucleic Acid Sequence/Function Analysis

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      Pages 99-112

      In Vivo Analyses of Viral RNA Translation

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      Pages 113-124

      In Vitro Analysis of Translation Enhancers

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      Pages 125-133

      Identification of Plant Virus IRES

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      Pages 135-143

      Analysis of Geminivirus DNA Replication by 2-D Gel

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      Pages 145-166

      Begomoviruses: Molecular Cloning and Identification of Replication Origin

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      Pages 167-183

      Analysis of Viroid Replication

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      Pages 185-200

      Biochemical Analyses of the Interactions Between Viral Polymerases and RNAs

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      Pages 201-216

      In Situ Detection of Plant Viruses and Virus-Specific Products

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      Pages 217-227

      Detection of siRNAs and miRNAs

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      Pages 229-241

      Cloning of Short Interfering RNAs from Virus-Infected Plants

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      Pages 243-250

      Solution Structure Probing of RNA Structures

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      Pages 251-264

      RNA Encapsidation Assay

  4. Protein Analysis and Investigation of Protein Function

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      Pages 267-277

      Surface Plasmon Resonance Analysis of Interactions Between Replicase Proteins of Tomato Bushy Stunt Virus

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      Pages 279-291

      Biochemical Approaches for Characterizing RNA–Protein Complexes in Preparation for High Resolution Structure Analysis

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