Plant Pathology

Techniques and Protocols

Editors:

ISBN: 978-1-58829-799-0 (Print) 978-1-59745-062-1 (Online)

Table of contents (24 protocols)

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  1. Front Matter

    Pages i-ix

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    Pages 1-13

    Bioassay for Diagnosis of Plant Viruses

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    Pages 15-25

    Development of Enzyme Linked, Tissue Blot and Dot Blot Immunoassays for Plant Virus Detection

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    Pages 27-35

    Immunisation strategies for Antibody Production

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    Pages 37-50

    Preparation of Immunogens and Production of Antibodies

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    Pages 51-62

    Enzyme-Linked Immunosorbent Assay Detection of Ralstonia solanacearum in Potatoes: The South African Experience

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    Pages 63-74

    Production of Monoclonal Antibodies to Plant Pathogens

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    Pages 75-87

    Enzyme-Linked Immunosorbent Assay for the Detection and Identification of Plant Pathogenic Bacteria (In Particular for Erwinia amylovora and Clavibacter michiganensis subsp. sepedonicus)

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    Pages 89-99

    Indirect Immunofluorescence Microscopy for the Detection and Identification of Plant Pathogenic Bacteria (In Particular for Ralstonia solanacearum)

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    Pages 101-114

    Detection of Four Major Bacterial Potato Pathogens

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    Pages 115-129

    Erwinia amylovora: Modern Methods for Detection and Differentiation

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    Pages 131-145

    The use of Fluorescent in situ Hybridisation in Plant Fungal Identification and Genotyping

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    Pages 147-159

    Use of Molecular Methods for the Detection of Fungal Spores

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    Pages 161-169

    Identification of Phytophthora fragariae var. rubi by PCR

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    Pages 171-182

    Detection of Double-Stranded RNA Elements in the Plant Pathogenic Fungus Rhizoctonia solani

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    Pages 183-192

    Immunocapture-PCR for Plant Virus Detection

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    Pages 193-208

    Multiplex Polymerase Chain Reaction (PCR) and Real-time Multiplex PCR for the Simultaneous Detection of Plant Viruses

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    Pages 209-234

    Fluorescent-based Techniques for Viral Detection, Quantification andCharacterisation

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    Pages 235-247

    Analysis of Population Structures of Viral Isolates using Single StrandConformation Polymorphism (SSCP) Method

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    Pages 249-258

    Direct Detection of Plant Viruses in Potato Tubers using Real-time PCR

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    Pages 259-265

    An Accelerated Soil Bait Assay for the Detection of Potato Mop Top Virus in Agricultural Soil

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