Immobilization of Enzymes and Cells

Editors:

ISBN: 978-1-58829-290-2 (Print) 978-1-59745-053-9 (Online)

Table of contents (37 protocols)

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  1. Front Matter

    Pages i-xiv

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    Pages 1-13

    Immobilization of Enzymes as the 21st Century Begins

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    Pages 15-30

    Immobilization of Enzymes

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    Pages 31-45

    Cross-Linked Enzyme Aggregates

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    Pages 47-55

    Immobilization-Stabilization of Enzymes by Multipoint Covalent Attachment on Supports Activated With Epoxy Groups

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    Pages 57-64

    Glutaraldehyde in Protein Immobilization

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    Pages 65-76

    Practical Protocols for Lipase Immobilization Via Sol-Gel Techniques

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    Pages 77-85

    Encapsulation of Enzymes Using Polymers and Sol-Gel Techniques

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    Pages 87-95

    Design of Smart Biocatalysts

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    Pages 97-106

    Affinity Immobilization of Tagged Enzymes

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    Pages 107-116

    Bioaffinity Immobilization

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    Pages 117-128

    One-Step Purification, Immobilization, and Stabilization of Poly-Histidine-Tagged Enzymes Using Metal Chelate-Epoxy Supports

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    Pages 129-141

    Stabilization of Multimeric Enzymes Via Immobilization and Further Cross-Linking With Aldehyde-Dextran

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    Pages 143-152

    Purification, Immobilization, Hyperactivation, and Stabilization of Lipases by Selective Adsorption on Hydrophobic Supports

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    Pages 153-162

    Immobilization and Stabilization of Proteins by Multipoint Covalent Attachment on Novel Amino-Epoxy-Sepabeads®

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    Pages 163-173

    Improved Stabilization of Chemically Aminated Enzymes Via Multipoint Covalent Attachment on Glyoxyl Supports

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    Pages 175-183

    Stabilization of New Imprint Property of Glucose Oxidase in Pure Aqueous Medium by Cross-Linked-Imprinting Approach

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    Pages 185-204

    Reversible Covalent Immobilization of Enzymes Via Their Thiol Groups

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    Pages 205-216

    Very Strong But Reversible Immobilization of Enzymes on Supports Coated With Ionic Polymers

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    Pages 217-228

    Immobilization of Enzymes on Magnetic Particles

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    Pages 229-238

    Immobilization of Proteins on Gold Surfaces

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