Book Volume 633 2010

Mouse Cell Culture

Methods and Protocols

Editors:

ISBN: 978-1-58829-772-3 (Print) 978-1-59745-019-5 (Online)

Table of contents (18 protocols)

  1. Front Matter

    Pages i-xi

  2. No Access

    Protocol

    Pages 1-18

    The Culture of Mouse Embryonic Stem Cells and Formation of Embryoid Bodies

  3. No Access

    Protocol

    Pages 19-27

    Derivation of Primary Mouse Embryonic Fibroblast (PMEF) Cultures

  4. No Access

    Protocol

    Pages 29-56

    Embryonic Skeletal Muscle Microexplant Culture and Isolation of Skeletal Muscle Stem Cells

  5. No Access

    Protocol

    Pages 57-69

    The Embryonic Kidney: Isolation, Organ Culture, Immunostaining and RNA Interference

  6. No Access

    Protocol

    Pages 71-79

    Explant Culture of Mouse Embryonic Whole Lung, Isolated Epithelium, or Mesenchyme Under Chemically Defined Conditions as a System to Evaluate the Molecular Mechanism of Branching Morphogenesis and Cellular Differentiation

  7. No Access

    Protocol

    Pages 81-90

    Isolation, Culture, and Characterisation of Mouse Embryonic Oesophagus and Intestine

  8. No Access

    Protocol

    Pages 91-99

    Isolation and Culture of Embryonic Pancreas and Liver

  9. No Access

    Protocol

    Pages 101-111

    Isolation and Culture of Mouse Satellite Cells

  10. No Access

    Protocol

    Pages 113-124

    Analysis of Cardiac Myocyte Biology in Transgenic Mice: A Protocol for Preparation of Neonatal Mouse Cardiac Myocyte Cultures

  11. No Access

    Protocol

    Pages 125-138

    Short- and Long-Term Cultivation of Embryonic and Neonatal Murine Keratinocytes

  12. No Access

    Protocol

    Pages 139-170

    Isolation, Culture and Analysis of Mouse Mammary Epithelial Cells

  13. No Access

    Protocol

    Pages 171-184

    Isolation and Culture of Mouse Pancreatic Islets for Ex Vivo Imaging Studies with Trappable or Recombinant Fluorescent Probes

  14. No Access

    Protocol

    Pages 185-196

    Isolation and Culture of Adult Mouse Hepatocytes

  15. No Access

    Protocol

    Pages 197-206

    Isolation and Culture of Mouse Intestinal Cells

  16. No Access

    Protocol

    Pages 207-220

    Derivation of Primary Choroid Plexus Epithelial Cells from the Mouse

  17. No Access

    Protocol

    Pages 221-231

    The Preparation of Primary Cortical Neuron Cultures and a Practical Application Using Immunofluorescent Cytochemistry

  18. No Access

    Protocol

    Pages 233-239

    Cell Culture of Primary Cerebellar Granule Cells

  19. No Access

    Protocol

    Pages 241-252

    Isolation and Generation of Neurosphere Cultures from Embryonic and Adult Mouse Brain

  20. Back Matter

    Pages 253-257