Xenopus Protocols

Cell Biology and Signal Transduction

Editors:

ISBN: 978-1-58829-362-6 (Print) 978-1-59745-000-3 (Online)

Table of contents (33 protocols)

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  1. Front Matter

    Pages i-xv

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    Pages 1-16

    Resources for Genetic and Genomic Studies of Xenopus

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    Pages 17-30

    The Physiology of the Xenopus laevis Ovary

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    Pages 31-41

    Oocyte Isolation and Enucleation

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    Pages 43-53

    Xenopus tropicalis Oocytes

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    Pages 55-67

    Oocyte Expression With Injection of Purified T7 RNA Polymerase

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    Pages 69-86

    Visualization of the Cytoskeleton in Xenopus Oocytes and Eggs by Confocal Immunofluorescence Microscopy

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    Pages 87-101

    Multiphoton Laser Scanning Microscopy as a Tool for Xenopus Oocyte Research

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    Pages 103-119

    Imaging Ca2+ Signals in Xenopus Oocytes

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    Pages 121-137

    Chromosomal DNA Replication in a Soluble Cell-Free System Derived From Xenopus Eggs

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    Pages 139-147

    Chromatin Assembly of DNA Templates Microinjected Into Xenopus Oocytes

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    Pages 149-163

    Pre-mRNA Splicing in the Nuclei of Xenopus Oocytes

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    Pages 165-181

    Chromatin Immunoprecipitation for Studying Transcriptional Regulation in Xenopus Oocytes and Tadpoles

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    Pages 183-198

    Cytoplasmic mRNA Polyadenylation and Translation Assays

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    Pages 199-212

    Xenopus Egg Extracts

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    Pages 213-222

    Analysis of Molecular Chaperones Using a Xenopus Oocyte Protein Refolding Assay

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    Pages 223-234

    Ubiquitin-Mediated Protein Degradation in Xenopus Egg Extracts

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    Pages 235-258

    Introduction to Nucleocytoplasmic Transport

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    Pages 259-272

    Use of Xenopus laevis Oocyte Nuclei and Nuclear Envelopes in Nucleocytoplasmic Transport Studies

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    Pages 273-288

    Nuclear Pore Complex Structure and Plasticity Revealed by Electron and Atomic Force Microscopy

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    Pages 289-300

    In Vitro Study of Nuclear Assembly and Nuclear Import Using Xenopus Egg Extracts

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