1998

Molecular Biomethods Handbook

Editors:

ISBN: 978-0-89603-501-0 (Print) 978-1-59259-642-3 (Online)

Table of contents (51 protocols)

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  1. Front Matter

    Pages i-xiii

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    Book Protocol

    Pages 1-8

    Extraction of Total RNA from Tissues and Cultured Cells

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    Book Protocol

    Pages 9-16

    Isolation of Total Cellular DNA from Tissues and Cultured Cells

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    Pages 17-33

    Gel Electrophoresis of DNA

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    Pages 35-49

    Sl Nuclease Analysis of RNA

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    Pages 51-57

    Detecting mRNA by Use of the Ribonuclease Protection Assay (RPA)

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    Pages 59-72

    Gene Probes

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    Pages 73-76

    Nonradioactive Labeling of DNA

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    Pages 77-87

    Southern Blot Analysis

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    Pages 89-93

    Northern Blot Analysis

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    Book Protocol

    Pages 95-108

    DNA Sequencing

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    Pages 109-119

    Autoradiography and Fluorography

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    Pages 121-129

    Mobility Shift Assays

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    Pages 131-143

    cDNA Libraries

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    Pages 145-152

    Genomic DNA Libraries

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    Pages 153-163

    λ as a Cloning Vector

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    Pages 165-179

    Plasmid-Derived Cloning Vectors

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    Pages 181-191

    M13 and Phagemid-Based Cloning Vectors

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    Pages 193-218

    Retroviral Vectors From Laboratory Tools to Molecular Medicines

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    Pages 219-233

    Baculovirus Vectors

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    Book Protocol

    Pages 235-250

    Gene Transfer and Expression in Tissue Culture Cells of Higher Eukaryotes

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