Volume 9 2000

Downstream Processing of Proteins

Methods and Protocols

Editors:

ISBN: 978-0-89603-564-5 (Print) 978-1-59259-027-8 (Online)

Table of contents (16 protocols)

  1. Front Matter

    Pages i-ix

  2. No Access

    Book Protocol

    Pages 1-10

    Downstream Processing in the Biotechnology Industry

  3. No Access

    Book Protocol

    Pages 11-21

    2 Microbial Cell Disruption by High-Pressure Homogenization

  4. No Access

    Book Protocol

    Pages 23-34

    3 Protein Concentration and Buffer Exchange Using Ultrafiltration

  5. No Access

    Book Protocol

    Pages 35-46

    4 High-Performance Tangential Flow Filtration for Protein Separations

  6. No Access

    Book Protocol

    Pages 47-58

    Large-Scale Recovery of Protein Inclusion Bodies by Continuous Centrifugation

  7. No Access

    Book Protocol

    Pages 59-72

    Application of Density Gradient Ultracentrifugation Using Zonal Rotors in the Large-Scale Purification of Biomolecules

  8. No Access

    Book Protocol

    Pages 73-94

    Application of Chromatography in the Downstream Processing of Biomolecules

  9. No Access

    Book Protocol

    Pages 95-107

    Affinity Ligand Technology

  10. No Access

    Book Protocol

    Pages 109-120

    Immunoaffinity Adsorption in the Large-Scale Isolation of Biomolecules

  11. No Access

    Book Protocol

    Pages 121-139

    Expanded Bed Adsorption in the Purification of Biomolecules

  12. No Access

    Book Protocol

    Pages 141-160

    Application of HPLC in the Purification of Biomolecules

  13. No Access

    Book Protocol

    Pages 161-172

    Countercurrent Chromatography of Proteins with Polymer Phase Systems

  14. No Access

    Book Protocol

    Pages 173-184

    Practical Aspects and Applications of Radial Flow Chromatography

  15. No Access

    Book Protocol

    Pages 185-194

    Sterile Filtration and Virus Filtration

  16. No Access

    Book Protocol

    Pages 195-209

    Design and Interpretation of Viral Clearance Studies for Biopharmaceutical Products

  17. No Access

    Book Protocol

    Pages 211-221

    Validation of Sterilizing Filters in the Biotechnology Industry

  18. Back Matter

    Pages 223-229