Volume 1151 2014

Engineering and Analyzing Multicellular Systems

Methods and Protocols

Editors:

ISBN: 978-1-4939-0553-9 (Print) 978-1-4939-0554-6 (Online)

Table of contents (16 protocols)

  1. Front Matter

    Pages i-xi

  2. Constructing Multicellular Systems

    1. Front Matter

      Pages 1-1

    2. No Access

      Book Protocol

      Pages 3-25

      Recent Progress in Engineering Human-Associated Microbiomes

    3. No Access

      Book Protocol

      Pages 27-38

      Constructing Synthetic Microbial Communities to Explore the Ecology and Evolution of Symbiosis

    4. No Access

      Book Protocol

      Pages 39-47

      Combining Engineering and Evolution to Create Novel Metabolic Mutualisms Between Species

    5. No Access

      Book Protocol

      Pages 49-68

      Design, Construction, and Characterization Methodologies for Synthetic Microbial Consortia

    6. No Access

      Book Protocol

      Pages 69-74

      An Observation Method for Autonomous Signaling-Mediated Synthetic Diversification in Escherichia coli

    7. No Access

      Book Protocol

      Pages 75-94

      Integration-Free Reprogramming of Human Somatic Cells to Induced Pluripotent Stem Cells (iPSCs) Without Viral Vectors, Recombinant DNA, and Genetic Modification

    8. No Access

      Book Protocol

      Pages 95-101

      Transformation of Bacillus subtilis

    9. No Access

      Book Protocol

      Pages 103-115

      Culturing Anaerobes to Use as a Model System for Studying the Evolution of Syntrophic Mutualism

    10. No Access

      Book Protocol

      Pages 117-133

      Therapeutic Microbes for Infectious Disease

  3. Analyzing and Modeling Multicellular Systems

    1. Front Matter

      Pages 135-135

    2. No Access

      Book Protocol

      Pages 137-149

      Quantitative Measurement and Analysis in a Synthetic Pattern Formation Multicellular System

    3. No Access

      Book Protocol

      Pages 151-164

      Transcriptome Analysis of a Microbial Coculture in which the Cell Populations Are Separated by a Membrane

    4. No Access

      Book Protocol

      Pages 165-188

      Identification of Mutations in Laboratory-Evolved Microbes from Next-Generation Sequencing Data Using breseq

    5. No Access

      Book Protocol

      Pages 189-197

      3D-Fluorescence In Situ Hybridization of Intact, Anaerobic Biofilm

    6. No Access

      Book Protocol

      Pages 199-208

      The Characterization of Living Bacterial Colonies Using Nanospray Desorption Electrospray Ionization Mass Spectrometry

    7. No Access

      Book Protocol

      Pages 209-231

      Modeling Community Population Dynamics with the Open-Source Language R

    8. No Access

      Book Protocol

      Pages 233-253

      Simulating Microbial Community Patterning Using Biocellion

  4. Back Matter

    Pages 255-256