1994

Handbook for Rhizobia

Methods in Legume-Rhizobium Technology

Authors:

ISBN: 978-1-4613-8377-2 (Print) 978-1-4613-8375-8 (Online)

Table of contents (38 chapters)

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  1. Front Matter

    Pages i-xvi

  2. General Microbiology of Rhizobia

    1. Front Matter

      Pages 1-6

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      Book Chapter

      Pages 7-23

      Collecting Nodules and Isolating Rhizobia

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      Book Chapter

      Pages 24-30

      Observing the Infection Process

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      Book Chapter

      Pages 31-37

      Cultural Properties, Cell Morphology, and Nutritional Requirements of Rhizobia

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      Book Chapter

      Pages 38-46

      Demonstrating Genetic Diversity in Rhizobia Using Patterns of Carbohydrate Utilization and Intrinsic Antibiotic Resistance

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      Book Chapter

      Pages 47-57

      Quantifying the Growth of Rhizobia

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      Book Chapter

      Pages 58-64

      Counting Rhizobia by a Plant Infection Method

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      Book Chapter

      Pages 65-74

      Counting Serologically Specific Rhizobia in Soil and Peat Inoculants Using Membrane Filters and Immunofluorescence

    9. Back Matter

      Pages 75-77

  3. Identification of Rhizobia

    1. Front Matter

      Pages 79-88

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      Book Chapter

      Pages 89-93

      Developing Antisera

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      Book Chapter

      Pages 94-101

      Somatic Agglutination Reactions with Pure Cultures of Rhizobia

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      Book Chapter

      Pages 102-106

      Agglutinating Antigens from Root Nodules

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      Book Chapter

      Pages 107-111

      Performing Rhizobial Antigen-Antibody Reactions by Gel Immunodiffusion

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      Book Chapter

      Pages 112-119

      Determining Strain Occupancy in Soybean Nodules by Gel Immunodiffusion

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      Book Chapter

      Pages 120-130

      Producing and Applying Fluorescent Antibodies

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      Book Chapter

      Pages 131-139

      Identifying Rhizobia by the Indirect Enzyme-Linked Immunosorbent Assay

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      Book Chapter

      Pages 140-148

      Identifying Rhizobia by Immunoblot

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      Book Chapter

      Pages 149-152

      Isolating Spontaneous Antibiotic-Resistant Mutants of Rhizobia

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      Book Chapter

      Pages 153-157

      Analyzing Nodule Occupancy Using Antibiotic-Resistant Markers

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      Book Chapter

      Pages 158-162

      Distinguishing between Strains of Rhizobia by Rhizobiophage Susceptibility

    13. Back Matter

      Pages 163-164

  4. Evaluating Symbiotic Potential of Rhizobia

    1. Front Matter

      Pages 165-169

    2. No Access

      Book Chapter

      Pages 171-176

      Testing For Genetic Compatibility between Rhizobia and Legumes

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      Book Chapter

      Pages 177-181

      Screening Rhizobia for Nitrogen-Fixation Potential

    4. Back Matter

      Pages 214-215

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