Volume 40 2006

Reviews and Protocols in DT40 Research

Subcellular Biochemistry

Editors:

ISBN: 978-1-4020-4895-1 (Print) 978-1-4020-4896-8 (Online)

Table of contents (43 chapters)

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  1. Front Matter

    Pages i-xii

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    Book Chapter

    Pages 1-9

    DT40 gene disruptions: a how-to for the design and the construction of targeting vectors

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    Pages 11-24

    Immunoglobulin gene conversion or hypermutation: that's the question

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    Pages 25-37

    Genome resources for the DT40 community

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    Pages 39-48

    Chromosome engineering in DT40 cells and mammalian centromere function

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    Pages 49-73

    Function of RECQ family helicase in genome stability

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    Pages 75-90

    Genetic analysis of apoptotic execution

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    Pages 91-106

    The DT40 system as a tool for analyzing kinetochore assembly

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    Pages 107-117

    Analysing the DNA damage and replication checkpoints in DT40 cells

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    Pages 119-143

    Using DT40 to study clathrin function

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    Pages 145-187

    Genetic analysis of B cell signaling

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    Pages 189-205

    DT40 mutants: a model to study transcriptional regulation of B cell development and function

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    Pages 207-223

    Transcription and RNA processing factors play complex roles in DT40 cells

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    Pages 225-243

    Participation of histones, histone modifying enzymes and histone chaperonesin vertebrate cell functions

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    Pages 245-256

    Analysis of gene expression, copy number and palindrome formation with a DT40 enriched CDNA microarray

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    Pages 257-270

    Calcium signaling, ion channels and more

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    Pages 271-294

    Analysis of DNA replication damage bypass and its role in immunoglobulin repertoire development

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    Pages 295-311

    The fanconi anemia pathway promotes homologous recombination repair in DT40 cell line

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    Pages 313-325

    Phenotypic analysis of cellular responses to DNA damage

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    Pages 327-339

    ATM, a paradigm for a stress-responsive signal transducer in higher vertebrate cells

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    Book Chapter

    Pages 341-344

    Stable non-targeted transfection of DT40

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