Homing Endonucleases and Inteins

ISBN: 978-3-540-25106-4 (Print) 978-3-540-29474-0 (Online)

Table of contents (20 chapters)

  1. Front Matter

    Pages I-XIX

  2. Chapter

    Pages 1-10

    Back to Basics: Structure, Function, Evolution and Application of Homing Endonucleases and Inteins

  3. Chapter

    Pages 11-31

    Homing Endonucleases and the Yeast Mitochondrial ω Locus — A Historical Perspective

  4. Chapter

    Pages 33-47

    The LAGLIDADG Homing Endonuclease Family

  5. Chapter

    Pages 49-65

    HNH Endonucleases

  6. Chapter

    Pages 67-83

    GIY-YIG Homing Endonucleases — Beads on a String

  7. Chapter

    Pages 85-102

    His-Cys Box Homing Endonucleases

  8. Chapter

    Pages 103-119

    Group I Introns and Their Maturases: Uninvited, but Welcome Guests

  9. Chapter

    Pages 121-145

    Group II Intron Homing Endonucleases: Ribonucleoprotein Complexes with Programmable Target Specificity

  10. Chapter

    Pages 147-160

    Free-Standing Homing Endonucleases of T-even Phage: Freeloaders or Functionaries?

  11. Chapter

    Pages 161-175

    Function and Evolution of HO and VDE Endonucleases in Fungi

  12. Chapter

    Pages 177-192

    Engineering Homing Endonucleases for Genomic Applications

  13. Chapter

    Pages 193-210

    Inteins — A Historical Perspective

  14. Chapter

    Pages 211-231

    Origin and Evolution of Inteins and Other Hint Domains

  15. Chapter

    Pages 233-255

    Biochemical Mechanisms of Intein-Mediated Protein Splicing

  16. Chapter

    Pages 257-271

    The Structure and Function of Intein-Associated Homing Endonucleases

  17. Chapter

    Pages 273-292

    Harnessing Inteins for Protein Purification and Characterization

  18. Chapter

    Pages 293-305

    Production of Cyclic Proteins and Peptides

  19. Chapter

    Pages 307-323

    Inteins for Split-Protein Reconstitutions and Their Applications

  20. Chapter

    Pages 325-344

    Intein Reporter and Selection Systems

  21. Chapter

    Pages 345-364

    Industrial Applications of Intein Technology

  22. Back Matter

    Pages 365-377