Volume 2 2005

Animal Cell Technology Meets Genomics

Proceedings of the 18th ESACT Meeting Granada, Spain, May 11–14, 2003

Editors:

ISBN: 978-1-4020-2791-8 (Print) 978-1-4020-3103-8 (Online)

Table of contents (165 chapters)

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  1. Cellular Mechanisms

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      Pages 3-13

      Effect of Osmotic Pressure on Gs-Ns0 Expression System

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      Pages 15-22

      Molecular Approaches to Influence Epigenetic Effectors of Transient and Stable Transgene Expression in Mammalian Cells

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      Pages 23-29

      Genomic Exploration on Chinese Hamster Ovary Cells

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      Pages 31-42

      Roles for Glycosylation in Receptor-Ligand Interactions in the Immune System

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      Pages 43-49

      Investigations on Mannose-6-Phosphate Receptor Mediated Protein Uptake

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      Pages 51-57

      Analysis of Multiple Apoptosis Parameters Using a Microfluidic Chip-Based System

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      Pages 59-65

      Inhibiting Apoptosis in Cell Culture Using Multiple Inhibitors

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      Pages 67-70

      Pausing of Mammalian Cells by Cold Exposure, Limits and Opportunities

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      Pages 71-74

      The Functional Competence of Animal Cells: Anal Ysis of the Secretory Pathway

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      Pages 75-78

      Proteome Analysis of Recombinant CHO Cells Under Hyperosmotic Stress

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      Pages 79-82

      Expression Profiling Analysis of Sodium Butyrate-Induced Chinese Hamster Ovary Cells in Defined Medium

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      Pages 83-86

      Intracellular Nucleotide Pools for Optimizing Product-Oriented Transient Transfection of Hek293 Cells in Suspension

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      Pages 87-89

      Impact of Yeast Pyruvate Carboxylase on the Productivity of Animal Host Cell Lines

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      Pages 91-94

      Environmental Effects of Lactate on High-Five™ Insect Cell Metabolism

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      Pages 95-98

      Correlation of Intracellular Nucleotide Pools to Amino Acid Concentrations in Culture Media by the Application of Multivariate Methods

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      Pages 99-102

      Engineering Trichoplusia ni (High-Five™) Insect Cells to Express a Cytosolic Pyruvate Carboxylase Enzyme Improves the Viability of the Cells in Batch, Fed-Batch and Perfusion Cultures

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      Pages 103-106

      Insect Cell Culture Medium Selection and Optimisation Based on Monitoring and Economical Considerations

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      Pages 107-110

      Antiapoptotic Activity of Synthetic and Nnatural Peptides

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      Pages 111-113

      Effect of Antiapoptotic Genes Expression on Cell Growth and Monoclonal Antibody Productivity in a Hybridoma Cell Line

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      Pages 115-120

      Amplified Dicistronic Expression Units Mediate Apoptosis Protection in CHO-DG44 Cells Adapted for Growth in Serum-Free Media, Impact on Mitochondria Copy Number

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