2002

Animal Cell Technology: Challenges for the 21st Century

Proceedings of the joint international meeting of the Japanese Association for Animal Cell Technology (JAACT) and the European Society for Animal Cell Technology (ESACT) 1998, Kyoto, Japan

Editors:

ISBN: 978-0-7923-5805-3 (Print) 978-0-306-46869-8 (Online)

Table of contents (76 chapters)

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  1. Asian Biotechnology in Future

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      Pages 1-4

      The Role of Hong Kong SAR in the Development of Biotechnology Industry in China

  2. Cell Culture and Engineering

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      Pages 5-9

      Increase in Intracellular Calcium Influx Enhances Monoclonal Antibody Productivity

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      Pages 11-15

      Energy Metabolism and Antibody Production Based on Flux Analysis in Hybridoma

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      Pages 17-22

      Transient Transfection Induces Early Cytosolic Calcium Signaling in CHO-K1 Cells

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      Pages 23-27

      An On-Line Feeding Strategy for Fed-Batch and Dialysis Cultures of Hybridoma Cells

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      Pages 29-32

      Effects of Serum and Other Medium Components on Suspension Culture of CHO Cells Producing Tissue Plasminogen Activator

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      Pages 33-38

      Effects of Osmotic Pressure on Metabolism of CHO Cells Producing Tissue Plasminogen Activator in Adhesion Culture Compared with that in Suspension Culture

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      Pages 39-43

      Cultivation of CHO Cells on Novel Microcarriers

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      Pages 45-49

      Bleeding Strategy for the Long-Term Perfusion Culture of Hybridoma

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      Pages 51-55

      Metabolic Flux Distributions in Hybridoma Cells at Different Metabolic States

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      Pages 57-61

      On-Line Detection of Metabolic Demand and Changes to it: Heat Flux in Continuous Cultures of Animal Cells

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      Pages 63-67

      Growth Stimulation of CHO Cells by Phospholipids in Serum-Free Culture

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      Pages 69-73

      A Kinetic Model of Macromolecular Metabolism in Batch and Fed-Batch Cultures of Hybridoma Cells Producing Monoclonal Antibody

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      Pages 75-79

      Ehancement of Antibody Productivity in the Near-Zero Specific Growth Rate During Glucose and Glutamine-Limited Fed-Batch Culture of Hybridoma Cells

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      Pages 81-85

      Long-Term Culture of CHO Cells on Porous Microcarriers in a Stirred Tank Equipped with a Modified Cell Retention System

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      Pages 87-91

      Continuous production and recovery of recombinant Ca++ binding receptor from HEK 293 cells using perfusion through a packed bed bioreactor

  3. Production of Biologicals

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      Pages 93-97

      Characterization of Recombinant Bovine Lactoperoxidase Produced by CHO Cells

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      Pages 99-103

      Expression of a Soluble αβ Heterodimeric T Cell Receptor in Animal Cells

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      Pages 105-109

      Posttranslational Modifications of Recombinant Von Willebrand Factor: Limitations and Experimental Improvement at High Yield Expression

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      Pages 111-115

      Incubation with Heparin as an Improved Harvest Method for High Titre Disabled Infectious Single Cycle (DISC) HSV-2 from Microcarrier Cultures

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