2002

New Developments and New Applications in Animal Cell Technology

Proceedings of the 15th ESACT Meeting

Editors:

ISBN: 978-0-7923-5016-3 (Print) 978-0-306-46860-5 (Online)

Table of contents (141 chapters)

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  1. Sessions on: Biosynthesis and Post-Translational Modifications of Recombinant Proteins, and Cell Physiology and Metabolic Engineering of Animal Cells

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      Book Chapter

      Pages 1-3

      Genetic Manipulation of the Mammalian Genome

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      Pages 5-23

      Engineering Glycosylation in Animal Cells

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      Pages 25-26

      Sessions on: Biosynthesis and Post-Translational Modifications of Recombinant Proteins, and Cell Physiology and Metabolic Engineering of Animal Cells

  2. Gene Expression

    1. Front Matter

      Pages 27-27

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      Pages 29-33

      Enhanced Recombinant Protein Expression in Insect Cells in the Presence of Dimethylsulfoxide (DMSO)

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      Pages 35-37

      Studies of the Hepatitis C Virus Recombinant Protein Production and Its Effects on SF-9 Insect Cells Metabolism

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      Pages 39-41

      The Human Multidrug Transporter (MDR1) Expressed in the Baculovirus-SF9 Insect Cell System

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      Pages 43-49

      Glutamine Synthetase Transfected Cells May Avoid Selection by Releasing Glutamine

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      Pages 51-53

      Study of Different Cell Culture Conditions for the Production of a “Reshaped” Mab in NSO Cells

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      Pages 55-57

      Increasing Monoclonal Antibody Productivity by Semicontinuous Substitution of Production Medium for Growth Medium

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      Pages 59-67

      Expression of Recombinant Human Insulin in Chinese Hamster Ovary Cells is Complicated by Intracellular Insulin-Degrading Enzymes

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      Pages 69-76

      High Yield Expression of Recombinant Plasma Factors: Use of Recombinant Endoprotease Derivatives In Vivo and In Vitro

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      Pages 77-79

      The Role of Cell Cycle in Determining Levels of Gene Expression in Cho Cells

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      Pages 81-83

      Heterogeneity within DHFR-Mediated Gene Amplified Population of Cho Cells Producting Chimeric Antibodies

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      Pages 85-92

      Immunofluorescence Detection of Recombinant Monoclonal Antibody as a Tool in the Characterization of Chinese Hamster Ovary Cell Lines

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      Pages 93-96

      Electrolyzed Reduced Water Which Can Scavenge Active Oxygen Species Supresses Cell Growth and Regulates Gene Expression of Animal Cells

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      Pages 97-99

      Differential Gene Expression of Cytochrome P450 in Immortalised Hepatocyte Cell Lines

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      Pages 101-103

      Post-Transcriptional Control of Recombinant Gene Expression: mRNA Retargeting and Regulation of Expression

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      Pages 105-112

      Transient Transfection in Mammalian Cells

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      Pages 113-116

      Large Scale Transient Gene Expression in Mammalian Cells

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      Pages 117-120

      Synergistic Enhancement of Transient Expression by Dioleoyl-Melittin (DOM) and Polyethylenimine (PEI) in Mammalian Cells in Suspensionculture

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