Insect Cell Culture: Fundamental and Applied Aspects

Editors:

ISBN: 978-0-7923-3403-3 (Print) 978-0-306-46850-6 (Online)

Table of contents (27 chapters)

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  1. Insect Cell Lines

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      Pages 3-11

      Development and characterization of insect cell lines

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      Pages 13-22

      New approaches to insect tissue culture

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      Pages 23-31

      Transgenic insect cells: mosquito cell mutants and the dihydrofolate reductase gene

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      Pages 33-41

      Insect cell physiology

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      Pages 43-56

      Insect cell cultivation: growth and kinetics

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      Pages 57-70

      Medium design for insect cell culture

  2. Viruses

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      Pages 73-93

      Baculovirus-insect cell interactions

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      Pages 95-110

      Replication patterns and cytopathology of cells infected with baculoviruses

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      Pages 111-123

      Construction of baculovirus recombinants

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      Pages 125-137

      Passage effect of virus infection in insect cells

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      Pages 139-144

      Post-translational modifications in insect cells

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      Pages 145-148

      Productivity of insect cells for recombinant proteins

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      Pages 149-159

      Chaperone and foldase coexpression in the baculovirus-insect cell expression system

  3. Engineering Aspects

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      Pages 163-171

      Shear sensitivity of insect cells

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      Pages 173-189

      Insect cell bioreactors

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      Pages 191-198

      Perfusion bioreactors for the production of recombinant proteins in insect cells

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      Pages 199-208

      Immobilization of insect cells

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      Pages 209-219

      Modelling baculovirus infection of insect cells in culture

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      Pages 221-229

      Scale up aspects of sparged insect-cell bioreactors

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      Pages 231-238

      Oxygen gradients in small and big sparged insect-cell bioreactors

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