Overview
Biology of microRNAs for use as tools
Implementation of these molecules to improve processes in the Biopharmceutical field
Methods to implement these molecules in the Bioprocess setting
Includes supplementary material: sn.pub/extras
Includes supplementary material: sn.pub/extras
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Table of contents(8 chapters)
About this book
Focused manuscript on the potential use/role of miRNAs in bioprocessing, specifically the production of complex proteins in mammalian cells. With that in mind I propose a draft list of topics/chapters along the following lines: Intro on CHO/bioprocessing/engineering challenges to set scene, Genomic organization, biogenesis and mode of action, Identifying miRNA targets: Computational prediction, transcriptomics, proteomices, UTR analysis, etc., miRNA expression in Chinese Hamster Ovary cells, Â miRNAs as engineering targets: pathway manipulation to impact bioprocess phenotypes, miRNAs as biomarkers, Detection methods: Northern, PCR, hybridization arrays, Next Gen Seq, Manipulation of expression in cultured cells: Transient/stable disregulation, Knockout.
Editors and Affiliations
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, National Institute for Cellular Biotechn, Dublin City University, Dublin, Ireland
Niall Barron
Bibliographic Information
Book Title: MicroRNAs as Tools in Biopharmaceutical Production
Editors: Niall Barron
DOI: https://doi.org/10.1007/978-94-007-5128-6
Publisher: Springer Dordrecht
eBook Packages: Biomedical and Life Sciences, Biomedical and Life Sciences (R0)
Copyright Information: Springer Science+Business Media Dordrecht 2012
Hardcover ISBN: 978-94-007-5127-9Published: 16 November 2012
Softcover ISBN: 978-94-007-9526-6Published: 14 December 2014
eBook ISBN: 978-94-007-5128-6Published: 15 November 2012
Edition Number: 1
Number of Pages: X, 126
Topics: Pharmaceutical Sciences/Technology, Genetic Engineering, Animal Genetics and Genomics