Optical Fluorescence Microscopy

1. Front Matter

   Pages i-xii

   PDF

2. Fundamentals of Optical Microscopy

   • Franco Quercioli

   Pages 1-36

3. The White Confocal: Continuous Spectral Tuning in Excitation and Emission

   • Rolf Borlinghaus

   Pages 37-54

4. Second/Third Harmonic Generation Microscopy

   • Shakil Rehman, Naveen K. Balla, Elijah Y. Y. Seng, Colin J. R. Sheppard

   Pages 55-74

5. Role of Scattering and Nonlinear Effects in the Illumination and the Photobleaching Distribution Profiles

   • Zeno Lavagnino, Francesca Cella Zanacchi, Alberto Diaspro

   Pages 75-84

6. New Analytical Tools for Evaluation of Spherical Aberration in Optical Microscopy

   • Isabel Escobar, Emilio Sánchez-Ortiga, Genaro Saavedra, Manuel Martínez-Corral

   Pages 85-99

7. Improving Image Formation by Pushing the Signal-to-Noise Ratio

   • Emiliano Ronzitti, Giuseppe Vicidomini, Francesca Cella Zanacchi, Alberto Diaspro

   Pages 101-110

8. Site-Specific Labeling of Proteins in Living Cells Using Synthetic Fluorescent Dyes

   • Gertrude Bunt

   Pages 111-130

9. Imaging Molecular Physiology in Cells Using FRET-Based Fluorescent Nanosensors

   • Fred S. Wouters

   Pages 131-152

10. Measuring Molecular Dynamics by FRAP, FCS, and SPT

    • Kevin Braeckmans, Hendrik Deschout, Jo Demeester, Stefaan C. De Smedt

    Pages 153-163

11. In Vitro–In Vivo Fluctuation Spectroscopies

    • M. Collini, L. D’Alfonso, M. Caccia, L. Sironi, M. Panzica, G. Chirico et al.

    Pages 165-181

12. Interference X-ray Diffraction from Single Muscle Cells Reveals the Molecular Basis of Muscle Braking

    • L. Fusi, E. Brunello, M. Reconditi, R. Elangovan, M. Linari, Y.-B. Sun et al.

    Pages 183-189

13. Low Concentration Protein Detection Using Novel SERS Devices

    • Gobind Das, Francesco Gentile, Maria Laura Coluccio, G. Cojoc, Federico Mecarini, Francesco De Angelis et al.

    Pages 191-210

14. Near Infrared Three-Dimensional Nonlinear Optical Monitoring of Stem Cell Differentiation

    • Uday K. Tirlapur, Clarence Yapp

    Pages 211-229

15. A Correlative Microscopy: A Combination of Light and Electron Microscopy

    • Umberto Fascio, Anna Sartori-Rupp

    Pages 231-238

16. Back Matter

    Pages 239-244

    PDF

In the last decade, fluorescence microscopy has evolved from a classical “retrospective” microscopy approach into an advanced imaging technique that allows the observation of cellular activities in living cells with increased resolution and dimensions. A bright new future has arrived as the nano era has placed a whole new array of tools in the hands of biophysicists who are keen to go deeper into the intricacies of how biological systems work. Following an introduction to the complex world of optical microscopy, this book covers topics such as the concept of white confocal, nonlinear optical microscopy, fluctuation spectroscopies, site-specific labeling of proteins in living cells, imaging molecular physiology using nanosensors, measuring molecular dynamics, muscle braking and stem cell differentiation.

• Cellular imaging
• Fluorescent dyes
• Nanosensors
• Spectroscopy
• Visual system
• Biological Microscopy

From the reviews:

“This book offers a recent approach to applications of microscopy for the examination of biological processes and brings you to an introduction to the complex world of optical microscopy. … should be available in all schools, research laboratories, community and university libraries. A good book for the scientists working in molecular and cell biology, biochemistry, medicine, pharmacology and biophysics.” (Samantha C. Karunarathna, Fungal Diversity, April, 2012)

• , Nanophysics Unit, The Italian Institute of Technology - II, Genova, Italy

  Alberto Diaspro

Policies and ethics