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  • Book
  • © 2012

Mucins

Methods and Protocols

  • Highlights the technical challenges while describing procedures
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 842)

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Table of contents (19 protocols)

  1. Front Matter

    Pages i-xi
  2. Gel-Forming and Cell-Associated Mucins: Preparation for Structural and Functional Studies

    • Julia R. Davies, Claes Wickström, David J. Thornton
    Pages 27-47
  3. Detecting, Visualising, and Quantifying Mucins

    • Ceri A. Harrop, David J. Thornton, Michael A. McGuckin
    Pages 49-66
  4. Mass Spectrometric Analysis of Mucin Core Proteins

    • Mehmet Kesimer, John K. Sheehan
    Pages 67-79
  5. Analysis of Assembly of Secreted Mucins

    • Malin E. V. Johansson, Gunnar C. Hansson
    Pages 109-121
  6. MUC1 Membrane Trafficking: Protocols for Assessing Biosynthetic Delivery, Endocytosis, Recycling, and Release Through Exosomes

    • Franz-Georg Hanisch, Carol L. Kinlough, Simon Staubach, Rebecca P. Hughey
    Pages 123-140
  7. Glycomic Work-Flow for Analysis of Mucin O-Linked Oligosaccharides

    • Catherine A. Hayes, Szilard Nemes, Samah Issa, Chunsheng Jin, Niclas G. Karlsson
    Pages 141-163
  8. Analysing Mucin Degradation

    • Stephen D. Carrington, Jane A. Irwin, Li Liu, Pauline M. Rudd, Elizabeth Matthews, Anthony P. Corfield
    Pages 191-215
  9. Assessment of Mucus Thickness and Production In Situ

    • Lena Holm, Mia Phillipson
    Pages 217-227
  10. Ex Vivo Measurements of Mucus Secretion by Colon Explants

    • Jenny K. Gustafsson, Henrik Sjövall, Gunnar C. Hansson
    Pages 237-243
  11. Studying Mucin Secretion from Human Bronchial Epithelial Cell Primary Cultures

    • Lubna H. Abdullah, Cédric Wolber, Mehmet Kesimer, John K. Sheehan, C. William Davis
    Pages 259-277
  12. Assessment of Intracellular Mucin Content In Vivo

    • Lucia Piccotti, Burton F. Dickey, Christopher M. Evans
    Pages 279-295
  13. Techniques for Assessment of Interactions of Mucins with Microbes and Parasites In Vitro and In Vivo

    • Yong H. Sheng, Sumaira Z. Hasnain, Chin Wen Png, Michael A. McGuckin, Sara K. Lindén
    Pages 297-312

About this book

Epithelial mucins are large complex cell surface and secreted glycoproteins produced by mucosal epithelial cells.  In, Mucins: Methods and Protocols expert researchers in the field detail many of the methods which are now commonly used to study Mucins. These include methods and techniques  for the best approaches to analysing each specific area of mucin biochemistry, physiology and biophysics before providing individual detailed experimental protocols together with troubleshooting and interpretation tips. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and practical, Mucins: Methods and Protocols is designed to be a useful resource for those entering the mucin field and to facilitate those already studying mucins to broaden their experimental approaches to understanding mucosal biology.

Editors and Affiliations

  • Mucosal Diseases Program, Mater Medical Research Institute, South Brisbane, Australia

    Michael A. McGuckin

  • Fac. Life Sciences, Wellcome Trust Centre for, University of Manchester, Manchester, United Kingdom

    David J. Thornton

Bibliographic Information

  • Book Title: Mucins

  • Book Subtitle: Methods and Protocols

  • Editors: Michael A. McGuckin, David J. Thornton

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-61779-513-8

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2012

  • Hardcover ISBN: 978-1-61779-512-1Published: 19 January 2012

  • Softcover ISBN: 978-1-4939-5886-3Published: 23 August 2016

  • eBook ISBN: 978-1-61779-513-8Published: 21 January 2012

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 329

  • Topics: Biochemistry, general, Proteomics

Buy it now

Buying options

eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access