Skip to main content
SpringerLink
Log in

Bone Research Protocols

  • Book
  • © 2012

Overview

Editors:
  1. Miep H. Helfrich

    1. Institute of Medical Sciences, University of Aberdeen, Aberdeen, United Kingdom

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Stuart H. Ralston

    1. Institute of Genetics and Molecular Medi, Rheumatic Diseases Unit, University of Edinburgh, Edinburgh, United Kingdom

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Contains a catalogue of protocols to assist researchers in the pursuit of mechanistic studies
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 816)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (38 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. Culture of Osteoblasts and Osteocytes

    1. Front Matter

      Pages 1-1
      Download chapter PDF

  3. Culture of osteoblasts and osteocytes

    1. Primary Human Osteoblast Cultures

      • Jane P. Dillon, Victoria J. Waring-Green, Adam M. Taylor, Peter J. M. Wilson, Mark Birch, Alison Gartland et al.
      Pages 3-18

    2. Osteoblast Isolation from Murine Calvaria and Long Bones

      • Astrid D. Bakker, Jenneke Klein-Nulend
      Pages 19-29

    3. Rat Osteoblast Cultures

      • Isabel R. Orriss, Sarah E. B. Taylor, Timothy R. Arnett
      Pages 31-41

    4. Isolation of Primary Avian Osteocytes

      • Cor M. Semeins, Astrid D. Bakker, Jenneke Klein-Nulend
      Pages 43-53

    5. Isolation of Mouse Osteocytes Using Cell Fractionation for Gene Expression Analysis

      • Christine Halleux, Ina Kramer, Cyril Allard, Michaela Kneissel
      Pages 55-66

    6. Studying Osteocyte Function Using the Cell Lines MLO-Y4 and MLO-A5

      • Jennifer Rosser, Lynda F. Bonewald
      Pages 67-81

    7. Isolation, Differentiation, and Characterisation of Skeletal Stem Cells from Human Bone Marrow In Vitro and In Vivo

      • Rahul S. Tare, Peter D. Mitchell, Janos Kanczler, Richard O. C. Oreffo
      Pages 83-99

  4. Culture of Osteoclasts

    1. Front Matter

      Pages 101-101
      Download chapter PDF

  5. Culture of osteoclasts

    1. Rodent Osteoclast Cultures

      • Isabel R. Orriss, Timothy R. Arnett
      Pages 103-117

    2. Isolation and Culture of Primary Chicken Osteoclasts

      • Patricia Collin-Osdoby, Philip Osdoby
      Pages 119-143

    3. Isolation and Purification of Rabbit Osteoclasts

      • Fraser P. Coxon, Michael J. Rogers, Julie C. Crockett
      Pages 145-158

    4. Generation of Human Osteoclasts from Peripheral Blood

      • Kim Henriksen, Morten A. Karsdal, Adam Taylor, Denise Tosh, Fraser P. Coxon
      Pages 159-175

    5. Osteoclast Formation in Mouse Co-cultures

      • Cecile Itzstein, Robert J. van ‘t Hof
      Pages 177-186

    6. RANKL-Mediated Osteoclast Formation from Murine RAW 264.7 cells

      • Patricia Collin-Osdoby, Philip Osdoby
      Pages 187-202

  6. Biochemical and Molecular Analysis of Bone Cells

    1. Front Matter

      Pages 203-203
      Download chapter PDF

  7. Biochemical and molecular analysis of bone cells

    1. Transfection of Osteoclasts and Osteoclast Precursors

      • Julie C. Crockett, David J. Mellis, Adam Taylor
      Pages 205-222

    2. Analysis of Signalling Pathways by Western Blotting and Immunoprecipitation

      • Aymen I. Idris
      Pages 223-232

    3. Analysis of Transcriptional Regulation in Bone Cells

      • Huilin Jin, Stuart H. Ralston
      Pages 233-247
Back to top

Keywords

About this book

Studies over the past decade have continued to bring tremendous advances to our understanding of bone biology. New pathways have been discovered and expanded our knowledge of the ways in which genes and gene products affect bone cells and thereby bone mass and bone strength. In Bone Research Protocols, Second Edition, expert researchers in the field detail many methods commonly used to study bone biology. Focusing mainly on in vitro methods, this volume gives techniques for isolation, culture and functional analysis of all bone cell types and details a range of imaging methods, including light and ultrastructural microscopy and live cell imaging. Some important in vivo techniques are included, such as analysis of bone resorption and imaging using X rays, fluorescent or luminescent techniques. Methods for study of proteins and nucleic acid are included and methods for analysis of bone composition, measurement of bone strength, and response to mechanical stimulation are described. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and practical, Bone Research Protocols, Second Edition seeks to aid scientists in the bone field to establish new techniques in their laboratories.

Reviews

From the reviews of the second edition:

“This volume presents detailed modern experimental methods to study bone biology. For bone researchers.” (Pediatric Endocrinology Reviews (PER), Vol. 9 (3), March, 2012)

Editors and Affiliations

  • Institute of Medical Sciences, University of Aberdeen, Aberdeen, United Kingdom

    Miep H. Helfrich

  • Institute of Genetics and Molecular Medi, Rheumatic Diseases Unit, University of Edinburgh, Edinburgh, United Kingdom

    Stuart H. Ralston

Bibliographic Information

  • Book Title: Bone Research Protocols

  • Editors: Miep H. Helfrich, Stuart H. Ralston

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-61779-415-5

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2012

  • Hardcover ISBN: 978-1-61779-414-8Published: 02 December 2011

  • Softcover ISBN: 978-1-4939-6100-9Published: 23 August 2016

  • eBook ISBN: 978-1-61779-415-5Published: 02 December 2011

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XIV, 607

  • Topics: Cell Biology, Developmental Biology, Animal Anatomy / Morphology / Histology

Publish with us

Policies and ethics

Back to top

Search

Navigation