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PCR Protocols

  • Book
  • © 2011

Overview

Editors:
  1. Daniel J. Park

    1. , Department of Pathology, The University of Melbourne, Parkville, Australia

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  • Presents detailed, independent methods, the core principles of which have broad and varied applications in the lab
  • Describes thoroughly the most recent and exciting fully developed protocols along with tips from the expert contributors
  • Inspires alternative approaches to aid all labs in producing successful results

Part of the book series: Methods in Molecular Biology (MIMB, volume 687)

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Table of contents (24 protocols)

  1. Front Matter

    Pages i-xi
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  2. PCR Husbandry

    1. Front Matter

      Pages 1-1
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    2. Setup of a PCR Laboratory

      • Zaheer Khan
      Pages 3-14

  3. Cloning and Sequencing

    1. Front Matter

      Pages 15-15
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    2. Long-Range PCR with a DNA Polymerase Fusion

      • Holly H. Hogrefe, Michael C. Borns
      Pages 17-23

    3. Isolation of Genomic Insertion Sites of Proviruses Using Splinkerette-PCR-Based Procedures

      • Bin Yin
      Pages 25-42

    4. Lariat-Dependent Nested PCR for Flanking Sequence Determination

      • Daniel J. Park
      Pages 43-55

    5. CODEHOP PCR and CODEHOP PCR Primer Design

      • Jeannette P. Staheli, Richard Boyce, Dina Kovarik, Timothy M. Rose
      Pages 57-73

    6. Sequencing of Difficult DNA Regions by SAM Sequencing

      • Keith R. Mitchelson
      Pages 75-88

  4. Expression, Epigenetic and Copy Number Analysis

    1. Front Matter

      Pages 89-89
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    2. A Global Single-Cell cDNA Amplification Method for Quantitative Microarray Analysis

      • Kazuki Kurimoto, Mitinori Saitou
      Pages 91-111

    3. Quantitation of MicroRNAs by Real-Time RT-qPCR

      • Caifu Chen, Ruoying Tan, Linda Wong, Richard Fekete, Jason Halsey
      Pages 113-134

    4. High-Throughput SuperSAGE

      • Hideo Matsumura, Kentaro Yoshida, Shujun Luo, Detlev H. Krüger, Günter Kahl, Gary P. Schroth et al.
      Pages 135-146

    5. Deep Cap Analysis of Gene Expression

      • Junpei Kurosawa, Hiromi Nishiyori, Yoshihide Hayashizaki
      Pages 147-163

    6. Linking Emulsion PCR Haplotype Analysis

      • James G. Wetmur, Jia Chen
      Pages 165-175

    7. PAP-LMPCR: An Improved, Sequence-Selective Method for the In Vivo Analysis of Transcription Factor Occupancy and Chromatin Fine Structure

      • Richard Ingram, Arthur Riggs, Constanze Bonifer
      Pages 177-192

    8. The Many Faces of MLPA

      • Thomas Ohnesorg, Erin Turbitt, Stefan J. White
      Pages 193-205

    9. Assessing Gene-Specific Methylation Using HRM-Based Analysis

      • Ee Ming Wong, Alexander Dobrovic
      Pages 207-217

  5. Probes and Assays

    1. Front Matter

      Pages 219-219
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    2. Alu PCR

      • Maurizio Cardelli
      Pages 221-229
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Keywords

About this book

Known for flexibility and robustness, PCR techniques continue to improve through numerous developments, including the identification of thermostable DNA polymerases which exhibit a range of properties to suit given applications. PCR Protocols, Third Edition selects recently developed tools and tricks, contributed by field-leading authors, for the significant value that they add to more generally established methods. Along with the cutting-edge methodologies, this volume describes many core applications, such as PCR cloning and sequencing, expression, copy number or methylation profile analysis, ‘DNA fingerprinting’, diagnostics, protein engineering, interaction screening as well as a chapter highlighting workflow considerations and contamination control, crucial for all PCR methods. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary reagents and materials, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, PCR Protocols, Third Edition seeks to further elucidate this essential technique while also providing core principles with broad applications for scientists of all backgrounds.

Reviews

From the reviews of the third edition:

“This book describes recently developed tools such as PCR cloning and sequencing, expression … and other important techniques crucial for all PCR methods. … Students and researchers interested in learning latest methods in PCR will find this book extremely useful. … This book presents cutting-edge methodologies crucial for all PCR methods. Given the rapid advances in the field of PCR methods, this third edition is essential and justifies replacement of the previous edition.” (Omer Iqbal, Doody’s Book Reviews, February, 2012)

“The book consists of 5 parts and 24 chapters. … Third edition of ‘PCR Protocols’ represents very useful handbook written in form of original and modified PCR protocols. Since it provides suggestions for solution of basic research as well as clinical and diagnostic problems, it will probably find its place in many molecular biologylaboratories.” (Ľubica Lacinová, General Physiology and Biochemistry, May, 2011)

Editors and Affiliations

  • , Department of Pathology, The University of Melbourne, Parkville, Australia

    Daniel J. Park

Bibliographic Information

  • Book Title: PCR Protocols

  • Editors: Daniel J. Park

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-60761-944-4

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2011

  • Hardcover ISBN: 978-1-60761-943-7Published: 28 October 2010

  • Softcover ISBN: 978-1-62703-819-5Published: 01 October 2014

  • eBook ISBN: 978-1-60761-944-4Published: 22 October 2010

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 3

  • Number of Pages: XI, 348

  • Topics: Human Genetics, Nucleic Acid Chemistry, Laboratory Medicine

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