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Principles of Fluorescence Spectroscopy

  • Textbook
  • © 1999

Overview

Authors:
  1. Joseph R. Lakowicz

    1. University of Maryland School of Medicine, Baltimore, USA

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Table of contents (22 chapters)

  1. Front Matter

    Pages i-xxv
    Download chapter PDF

  2. Introduction to Fluorescence

    • Joseph R. Lakowicz
    Pages 1-23

  3. Instrumentation for Fluorescence Spectroscopy

    • Joseph R. Lakowicz
    Pages 25-61

  4. Fluorophores

    • Joseph R. Lakowicz
    Pages 63-93

  5. Time-Domain Lifetime Measurements

    • Joseph R. Lakowicz
    Pages 95-140

  6. Frequency-Domain Lifetime Measurements

    • Joseph R. Lakowicz
    Pages 141-184

  7. Solvent Effects on Emission Spectra

    • Joseph R. Lakowicz
    Pages 185-210

  8. Dynamics of Solvent and Spectral Relaxation

    • Joseph R. Lakowicz
    Pages 211-236

  9. Quenching of Fluorescence

    • Joseph R. Lakowicz
    Pages 237-265

  10. Advanced Topics in Fluorescence Quenching

    • Joseph R. Lakowicz
    Pages 267-289

  11. Fluorescence Anisotropy

    • Joseph R. Lakowicz
    Pages 291-319

  12. Time-Dependent Anisotropy Decays

    • Joseph R. Lakowicz
    Pages 321-345

  13. Advanced Anisotropy Concepts

    • Joseph R. Lakowicz
    Pages 347-366

  14. Energy Transfer

    • Joseph R. Lakowicz
    Pages 367-394

  15. Time-Resolved Energy Transfer and Conformational Distributions of Biopolymers

    • Joseph R. Lakowicz
    Pages 395-424

  16. Energy Transfer to Multiple Acceptors, in One, Two, or Three Dimensions

    • Joseph R. Lakowicz
    Pages 425-443

  17. Protein Fluorescence

    • Joseph R. Lakowicz
    Pages 445-486

  18. Time-Resolved Protein Fluorescence

    • Joseph R. Lakowicz
    Pages 487-514

  19. Excited-State Reactions

    • Joseph R. Lakowicz
    Pages 515-530

  20. Fluorescence Sensing

    • Joseph R. Lakowicz
    Pages 531-572
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Keywords

About this book

`In the second edition of Principles I have attempted to maintain the emphasis on basics, while updating the examples to include more recent results from the literature. There is a new chapter providing an overview of extrinisic fluorophores. The discussion of timeresolved measurements has been expanded to two chapters. Quenching has also been expanded in two chapters. Energy transfer and anisotropy have each been expanded to three chapters. There is also a new chapter on fluorescence sensing. To enhance the usefulness of this book as a textbook, most chapters are followed by a set of problems. Sections which describe advanced topics are indicated as such, to allow these sections to be skipped in an introduction course. Glossaries are provided for commonly used acronyms and mathematical symbols. For those wanting additional informtion, the final appendix contains a list of recommended books which expand on various specialized topics.'
from the author's Preface

Reviews

about the first edition:
`Lakowicz's Principles of Fluorescence Spectroscopy has been the best one-volume introduction to the biophysical principles of fluorescence methods. Now that it has been updated and expanded with new coverage of fluorescent probes and their applications to molecular cell biology and clinical chemistry, it is likely to be the benchmark textbook on fluorescence technology.'
Roger Y. Tsien, Ph.D., University of California, San Diego
`Principles of Fluorescence Spectroscopy is encyclopedic and comprehensive ...'
Britton Chance, University of Pennsylvania, Philadelphia

Authors and Affiliations

  • University of Maryland School of Medicine, Baltimore, USA

    Joseph R. Lakowicz

About the author

Dr. J.R. Lakowicz is Professor of Biochemistry at the University of Maryland School of Medicine and Director of the Center for Fluorescence Spectroscopy. Dr. Lakowicz has published over 400 scientific articles, has edited numerous books, holds 16 issued patents, and is the sole author of the widely used text, Principles of Fluorescence Spectroscopy, also published by Kluwer Academic/Plenum Publishers, now in its Second Edition.

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