Bioactive Material Article

Journal of the Korean Society for Applied Biological Chemistry

, Volume 53, Issue 1, pp 33-38

Antioxidant and antimicrobial activities of extracts from a medicinal plant, sea buckthorn

  • Jong Hyun JeongAffiliated withCollege of Bioscience & Biotechnology, Kangwon National University
  • , Ji Won LeeAffiliated withCollege of Bioscience & Biotechnology, Kangwon National University
  • , Kyoung Su KimAffiliated withDepartment of Agricultural Biotechnology, Seoul National University
  • , Ju-Sung KimAffiliated withOriental Bio-herb Research Institute, Kangwon National University
  • , Sang No HanAffiliated withSamsung Herb Medicine Co., Ltd
  • , Chang Yeon YuAffiliated withCollege of Bioscience & Biotechnology, Kangwon National University
  • , Ju Kyong LeeAffiliated withCollege of Bioscience & Biotechnology, Kangwon National University
  • , Yong Soo KwonAffiliated withCollege of Pharmacy Kangwon National University
  • , Myong Jo KimAffiliated withCollege of Bioscience & Biotechnology, Kangwon National UniversityOriental Bio-herb Research Institute, Kangwon National University Email author 

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Abstract

This study was performed to evaluate the antioxidant and antimicrobial activities from the methanol extracts of the root and stem of Hippophae rhamnoides and their further partitioned fractions including hexane, ethyl acetate, butanol, and water. Antioxidant activities of the plant parts were measured by l,l-dephenyl-2-picryl-hydrazyl (DPPH), superoxide dismutase (SOD)-like activity, and ferric thiocyanate (FTC) assays, and compared to standard antioxidants of butylated hydroxyl anisole, butylated hydroxyl toluene, a-tocopherol, and ascorbic acid. Antimicrobial activity of the plant extracts and fractions were evaluated by determining the minimum inhibitory concentration values. DPPH assay showed that the overall strong antioxidant activities from the methanol extracts and fractions. The values of SOD-like activities in hexane fractions of the root and stem were close to the measurement of a reference, ascorbic acid at 1,000 ppm. The methanol extract of the root in FTC assay showed a remarkable antioxidant and free radical scavenging activity. The extracts and fractions of the root and stem showed better antimicrobial activity than compared antimicrobial agents, (+)-catechin, ketoconazol, and mycostantin. This study indicates that the plant root and stem contain a variety of compounds contributing to antioxidant and antimicrobial activity, which could be used for food additives and the development of useful natural compounds.

Key words

antimicrobial activity antioxidant activity DPPH FTC Hippophae rhamnoides ROS SOD