Inhibitory effect of citrus peel extract on lipid accumulation of 3T3-L1 adipocytes

  • Hee Kyung Jung
  • Yoo Seok Jeong
  • Chi-Duck Park
  • Chang-Ho Park
  • Joo-Heon Hong
Bioactive Meterials Article

DOI: 10.3839/jksabc.2011.028

Cite this article as:
Jung, H.K., Jeong, Y.S., Park, CD. et al. J. Korean Soc. Appl. Biol. Chem. (2011) 54: 169. doi:10.3839/jksabc.2011.028

Abstract

Inhibitory effects of lipid accumulation by citrus peel extract were evaluated. Citrus peel extract (CPE) (500 μg/mL) inhibited lipid and triglyceride accumulation in 3T3-L1 adipocytes by more than 64.5 and 58.7%, respectively and suppressed glycerol-3-phosphate dehydrogenase to the levels of preadipocytes. The expression of perilipin mRNA in 3T3-L1 adipocytes treated with CPE was significantly lower than that in untreated adipocytes (p<0.05), with no accompanying increase in lipolysis-related mRNA. Flavonoid compositions of CPE were hesperidin 13.79, narirutin 7, and naringin 262.5 μg/g, respectively. These findings suggest CPE may indirectly stimulate lipolysis by inhibiting protection of physical barrier on lipid droplet to hinder lipid accumulation in 3T3-L1 adipocytes.

Key words

adipocytecitrus peel extracthesperidinperilipinreal time-polymerase chain reaction

Copyright information

© Springer-Verlag 2003

Authors and Affiliations

  • Hee Kyung Jung
    • 1
  • Yoo Seok Jeong
    • 1
  • Chi-Duck Park
    • 1
  • Chang-Ho Park
    • 1
  • Joo-Heon Hong
    • 2
  1. 1.Bio Industry CenterDaegu TechnoparkDaeguRepublic of Korea
  2. 2.Department of Food Science and TechnologyCatholic University of DaeguGyeongsanRepublic of Korea