Journal of NeuroVirology

, Volume 15, Issue 5, pp 390–400

Measurement of soluble inflammatory mediators in cerebrospinal fluid of human immunodeficiency virus-positive patients at distinct stages of infection by solid-phase protein array


    • Department of NeurologyHeinrich-Heine-University, University Hospital Düsseldorf
  • Antje Lindecke
    • Biomedical Research Center (BMFZ)Heinrich-Heine-University
  • Eleni Koutsilieri
    • Department of Virology and ImmunobiologyUniversity of Würzburg
  • Matthias Maschke
    • Department of NeurologyUniversity Hospital of Duisburg-Essen, University of Duisburg-Essen
  • Ingo-W. Husstedt
    • Department of NeurologyUniversity of Münster
  • Sieghart Sopper
    • German Primate Center
  • Olaf Stüve
    • Department of NeurologyHeinrich-Heine-University, University Hospital Düsseldorf
    • University of Texas, Southwestern Medical Center
  • Hans-Peter Hartung
    • Department of NeurologyHeinrich-Heine-University, University Hospital Düsseldorf
  • Gabriele Arendt
    • Department of NeurologyHeinrich-Heine-University, University Hospital Düsseldorf
  • Competence Network HIV/AIDS

DOI: 10.3109/13550280903350192

Cite this article as:
Nolting, T., Lindecke, A., Koutsilieri, E. et al. Journal of NeuroVirology (2009) 15: 390. doi:10.3109/13550280903350192


The objective of this study was to evaluate immune cytokine expression in cerebrospinal fluid (CSF) of patients with human immunodeficiency virus-1 (HIV-1)-associated dementia (HAD) using a novel cytokine array assay. HIV-1 induces a condition resembling classical subcortical dementia, known as HAD. The immune mechanisms contributing to HAD have not been elucidated. Cytokine expression in CSF was determined by solid-phase protein array in 33 neurologically asymptomatic HIV-positive male patients and were compared to levels in non-HIV controls and patients with HAD. Neurological examinations and lumbar and venous punctures were conducted in all patients and controls. Interleukin (IL)-1, IL-4, and IL-10, were up-regulated in all treated acquired immunodeficiency syndrome (AIDS) patients independent of neurological status compared to controls. In contrast, interferon gamma (IFN-γ), IL-1α, IL-15, and tumor necrosis factor alpha (TNF-α) were highly expressed in patients with HAD compared to undemented HIV-positive patients. These results show that solid-phase protein array can detect immunological changes in patients infected with HIV. Cytokine expression levels differ in different disease stages and in patients on different treatment paradigms. Pending further validation on a larger number of patients, this method may be a useful tool in CSF diagnostics and the longitudinal evaluation of patient with HAD.


chemokinecytokineHIVneuro-AIDSprotein array

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© Journal of NeuroVirology, Inc. 2009