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Association of catalase (rs7943316) and glutathione peroxidase-1 (rs1050450) polymorphisms with the risk of type 2 diabetes (T2DM)

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Abstract

Reactive oxygen species are involved in the pathogenesis of type 2 diabetes mellitus (T2DM). Catalase and Glutathione peroxidase-1 are antioxidant enzymes and the activity of them is essential for the protection against damage caused by reactive oxygen species. The aim of this study was to investigate the association between genetic polymorphisms of CAT gene (rs7943316) and GPx-1 gene (rs1050450) in patients with type 2 diabetes. In this case-control study, a total of 120 Iranian patients with T2DM and 120 healthy individuals as control were included. Genotypes were determined by PCR-RFLP. A significant difference was found between genotyping distribution of CAT (–21 A/T) polymorphism.The frequency of TT genotype was increased in patients compared to controls and we observed a statistically significant difference (OR = 1.797, 95% CI = 0.975–3.318, P = 0.044). Distribution of genotypes did not differ for GPx-1 (198 C/T) polymorphism between the cases and controls subjects. This study indicated that CAT rs7943316 genotype (TT) was associated with an increased risk of T2DM. No evidence was found to support an association between GPx-1 (198 C/T) polymorphism and T2DM.

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References

  1. Hua, Y., Shen, J., Song, Y., Xing, Y., and Ye, X., Interleukin-10–592C/A,–819C/T and–1082A/G polymorphisms with risk of type 2 diabetes mellitus: A HuGE review and meta-analysis, PLoS One, 2013, vol. 8, p. e66568.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  2. Wild, S., Roglic, G., Green, A., Sicree, R., and King, H., Global prevalence of diabetes: Estimates for the year 2000 and projections for 2030, Diabetes Care, 2004, vol. 27, pp. 1047–1053.

    Article  PubMed  Google Scholar 

  3. Esteghamati, A., Gouya, M.M., Abbasi, M., et al., Prevalence of diabetes and impaired fasting glucose in the adult population of Iran: National Survey of Risk Factors for Non-Communicable Diseases of Iran, Diabetes Care, 2008, vol. 31, pp. 96–98.

    Article  PubMed  Google Scholar 

  4. Staiger, H., Machicao, F., Stefan, N., et al., Polymorphisms within novel risk loci for type 2 diabetes determine beta-cell function, PLoS One, 2007, vol. 2, p. e832.

    Article  PubMed Central  PubMed  Google Scholar 

  5. Tang, L., Wang, L., Liao, Q., et al., Genetic associations with diabetes: Meta-analyses of 10 candidate polymorphisms, PLoS One, 2013, vol. 8, p. e70301.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  6. Cavini, I., Catani, M.V., Evangelista, D., Gasperi, V., and Avigliano, L., Obesity-associated oxidative stress: strategies finalized to improve redox state, Int. J. Mol. Sci., 2013, vol. 14, pp. 10497–10538.

    Article  Google Scholar 

  7. Matough, F.A., Budin, S.B., Hamid, Z.A., Alwahaibi, N., and Mohamed, J., The role of oxidative stress and antioxidants in diabetic complications, Sultan Qaboos Univ. Med. J., 2012, vol. 12, p. 5.

    Article  PubMed Central  PubMed  Google Scholar 

  8. Kirkman, H.N. and Gaetani, G.F., Catalase: A tetrameric enzyme with four tightly bound molecules of NADPH, Proc. Natl. Acad. Sci. USA, 1984, vol. 81, pp. 4343–4347.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  9. Flekac, M., Skrha, J., Hilgertova, J., Lacinova, Z., and Jarolimkova, M., Gene polymorphisms of superoxide dismutases and catalase in diabetes mellitus, BMC Med. Genet., 2008, vol. 9, p. 30.

    Article  PubMed Central  PubMed  Google Scholar 

  10. Vitai, M., Fatrai, S., Rass, P., Csordas, M., and Tarnai, I., Simple PCR heteroduplex, SSCP mutation screening methods for the detection of novel catalase mutations in Hungarian patients with type 2 diabetes mellitus, Clin. Chem. Lab. Med., 2005, vol. 43, pp. 1346–1350.

    Article  CAS  PubMed  Google Scholar 

  11. Lubos, E., Loscalzo, J., and Handy, D.E., Glutathione peroxidase-1 in health and disease: From molecular mechanisms to therapeutic opportunities, Antioxid. Redox Signaling, 2011, vol. 15, pp. 1957–1997.

    Article  CAS  Google Scholar 

  12. Goldberg, M., Alberts, D.S., Buckmeier, J.A., Prasad, A.R., Krouse, R.S., and Diamond, A.M., Loss of heterozygosity at the glutathione peroxidase 1 locus is not an early event in colon carcinogenesis, Genes Cancer, 2011, vol. 2, pp. 910–913.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  13. Çelik, V.K., Sahin, Z.D., Sari, I., and Bakir, S., Comparison of oxidant/antioxidant, detoxification systems in various tissue homogenates and mitochondria of rats with diabetes induced by streptozocin, Exp. Diabetes Res., 2012, vol. 2012, id. 386831. doi: 10.1155/2012/386831

  14. Zhang, D.X. and Gutterman, D.D., Mitochondrial reactive oxygen species-mediated signaling in endothelial cells, Am. J. Physiol.: Heart Circ. Physiol., 2007, vol. 292, pp. H2023–H2031.

    CAS  Google Scholar 

  15. Da Ros, R., Assaloni, R., and Ceriello, A., Molecular targets of diabetic vascular complications and potential new drugs, Curr. Drug Targets, 2005, vol. 6, pp. 503–509.

    Article  PubMed  Google Scholar 

  16. Valko, M., Leibfritz, D., Moncol, J., Cronin, M.T., Mazur, M., and Telser, J., Free radicals and antioxidants in normal physiological functions and human disease, Int. J. Biochem. Cell Biol., 2007, vol. 39, pp. 44–84.

    Article  CAS  PubMed  Google Scholar 

  17. Rosen, P., Nawroth, P.P., King, G., Moller, W., Tritschler, H.J., and Packer, L., The role of oxidative stress in the onset and progression of diabetes and its complications: A summary of a Congress Series sponsored by UNESCO-MCBN, the American Diabetes Association and the German Diabetes Society, Diabetes/Metab. Res. Rev., 2001, vol. 17, pp. 189–212.

    Article  CAS  Google Scholar 

  18. Hamanishi, T., Furuta, H., Kato, H., et al., Functional variants in the glutathione peroxidase-1 (GPx-1) gene are associated with increased intima-media thickness of carotid arteries and risk of macrovascular diseases in Japanese type 2 diabetic patients, Diabetes, 2004, vol. 53, pp. 2455–2460.

    Article  CAS  PubMed  Google Scholar 

  19. Komina, A.V., Korostileva, K.A., Gyrylova, S.N., Belonogov, R.N., and Ruksha, T.G., Interaction between single nucleotide polymorphism in catalase gene and catalase activity under the conditions of oxidative stress, Physiol. Res., 2012, vol. 61, pp. 655–658.

    CAS  PubMed  Google Scholar 

  20. Quan, F., Korneluk, R.G., MacLeod, H.L., Tsui, L.C., and Gravel, R.A., An RFLP associated with the human catalase gene, Nucleic Acids Res., 1985, vol. 13, p. 8288.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  21. Panduru, N.M., Mota, E., Mota, M., Cimponeriu, D., Serafinceanu, C., and Cheta, D.M., Polymorphism of catalase gene promoter in Romanian patients with diabetic kidney disease and type 1 diabetes, Rom. J. Intern. Med., 2010, vol. 48, pp. 81–88.

    CAS  PubMed  Google Scholar 

  22. Vialykh, E.K., Solidolova, M.A., Bushueva, O., Bulgakova, I.V., and Polonikov, A.V., Catalase gene polymorphism is associated with increased risk of cerebral stroke in hypertensive patients, Zh. Nevrol. Psikhiatr. im. S.S. Korsakova, 2012, vol. 112, pp. 3–7.

    CAS  PubMed  Google Scholar 

  23. Tang, T., Prior, S., Li, K., et al., Association between the rs1050450 glutathione peroxidase-1 (C>T) gene variant and peripheral neuropathy in two independent samples of subjects with diabetes mellitus, Nutr., Metab. Cardiovasc. Dis., 2012, vol. 22, pp. 417–425.

    Article  CAS  Google Scholar 

  24. Atli, T., Keven, K., Avci, A., et al., Oxidative stress and antioxidant statusin elderly diabetes mellitus and glucose intolerance patients, Arch. Gerontol. Geriatr., 2004, vol. 39, pp. 269–275.

    Article  CAS  PubMed  Google Scholar 

  25. Men, T., Zhang, X., Yang, J., et al., The rs1050450 C>T polymorphism of GPX1 is associated with the risk of bladder but not prostate cancer: evidence from a metaanalysis, Tumor Biol., 2014, vol. 35, pp. 269–275.

    Article  CAS  Google Scholar 

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Correspondence to H. R. Miri.

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Saravani, S., Miri, H.R., Saravani, R. et al. Association of catalase (rs7943316) and glutathione peroxidase-1 (rs1050450) polymorphisms with the risk of type 2 diabetes (T2DM). Mol. Genet. Microbiol. Virol. 30, 216–220 (2015). https://doi.org/10.3103/S0891416815040096

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