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Effect of glyprolines PGP and N-acetyl-PGP on the expression of cytokine genes during stress- and acetate-induced ulceration

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Abstract

We have studied the effects of glyprolines PGP and N-acetyl-PGP on cytokine gene expression during stress and acetate-induced ulceration. Levels of RNA in mononuclear cells in circulating blood were evaluated using reverse transcription polymerase chain reaction. Intranasal administration of PGP (3.7 μmol/kg) has had a significant protective effect against stress-induced (59.4%) and acetate-induced (78.5%) ulceration in rats. N-acetyl-PGP has not changed the area of stress-induced damage, but it tended to inhibit the development of acetate ulcers. We found that stress-induced damage of gastric mucosa is accompanied by an increase in TNFα transcription and a decrease in IL-4 transcription. The development of acetate ulcers is accompanied by a decrease in expression of a number of cytokines: IFNα, IFNγ, IL-2, IL-4, IL-6, IL-12, and TNFα. The protective effect of PGP is accompanied by an increase in expression of IL-6. When N-acetyl-PGP is administered, an increase in the expression of Th-1 and Th-2 cytokines have been observed: IFNα, IFNγ, and IL-4.

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Authors and Affiliations

  1. Department of Biology, Moscow State University, 119991, Moscow, Russia

    A. D. Sangadzhieva & G. E. Samonina

  2. Pirogov Russian National Research Medical University, ul. Ostrovityanova 1, Moscow, 117997, Russia

    Z. V. Bakaeva

  3. Ivanovskii Research Institute of Virology, Moscow, Russia

    M. V. Mezentseva

  4. Institute of Molecular Genetics, Russian Academy of Sciences, pl. Akademika Kurchatova 2, Moscow, 123182, Russia

    L. A. Andreeva & N. F. Myasoedov

Authors
  1. A. D. Sangadzhieva
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  2. Z. V. Bakaeva
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  3. G. E. Samonina
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  4. M. V. Mezentseva
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  5. L. A. Andreeva
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  6. N. F. Myasoedov
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Corresponding author

Correspondence to A. D. Sangadzhieva.

Additional information

Original Russian Text © A.D. Sangadzhieva, Z.V. Bakaeva, G.E. Samonina, M.V. Mezentseva, L.A. Andreeva, N.F. Myasoedov, 2014, published in Vestnik Moskovskogo Universiteta. Biologiya, 2014, No. 4, pp. 3–8.

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Sangadzhieva, A.D., Bakaeva, Z.V., Samonina, G.E. et al. Effect of glyprolines PGP and N-acetyl-PGP on the expression of cytokine genes during stress- and acetate-induced ulceration. Moscow Univ. Biol.Sci. Bull. 69, 141–146 (2014). https://doi.org/10.3103/S0096392514040105

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  • DOI: https://doi.org/10.3103/S0096392514040105

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