Skip to main content
SpringerLink
Log in

A paradoxical effect of hydrated C60-fullerene at an ultralow concentration on the viability and aging of cultured Chinese hamster cells

  • Gerontology
  • Published:
Moscow University Biological Sciences Bulletin Aims and scope Submit manuscript

Abstract

The effect of an aqueous solution of hydrated C60-fullerene (HyFn) on the growth and “stationary phase aging” (accumulation of “age-related” changes in cultured cells during the slowing down of their proliferation within a single passage and the subsequent “aging” in the stationary phase of growth) of transformed B11-dii FAF28 Chinese hamster cells was studied. The final calculated concentration of HyFn in the growth medium was 10−19 M. A paradoxical result contrasting the available data on the absence of HyFn cytotoxicity at higher concentrations was obtained in our experiments: namely, HyFn decelerated cell proliferation (estimated by the growth of mass culture, as well as by the efficiency of colony formation) and accelerated the “stationary phase aging” of the cell culture. Moreover, repeated addition of an aqueous solution of HyFn (to the final calculated concentration of 10−19 M) to the cells that had already reached the stationary phase of growth caused a rapid (within no more than 24 h) death of a significant part of the cell population. The observed effect of HyFn at ultralow concentration is supposed to arise from the alterations in the properties of the water surrounding the fullerene molecule: namely, water becomes a donor and acceptor of electrons and regulates redox processes (especially those involving oxygen) in aqueous systems. This effect of HyFn at an ultralow concentration may be specific for transformed cells, and, therefore, experiments on normal fibroblasts with limited mitotic potential are planned as a continuation of the present study. It is also possible that the reported antiaging effect of HyFn in experimental animals is due to its anticancer, immunostimulatory, antiviral, and antibacterial properties manifested only at the whole-organism level.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. Andrievsky, G.V., Kosevich, M.V., Vovk, O.M., Shelkovsky, V.S., and Vashchenko, L.A., Are Fullerenes Soluble in Water?, in Recent Advances in the Chemistry and Physics of Fullerenes and Related Materials, Ruoff, R.S. and Kadish, K.M., Eds., Reno: Electrochem. Soc. Inc., 1995, pp. 1591–1602.

    Google Scholar 

  2. Andrievsky, G.V., Klochkov, V.K., Bordyuh, A., and Dovbeshko, G.I., Comparative Analysis of Two Aqueous-Colloidal Solutions of C-60 Fullerene with Help of FTIP Reflectance and UV-Vis Spectroscopy, Chem. Phys. Lett., 2002, vol. 364, no. 1, pp. 8–17.

    Article  CAS  Google Scholar 

  3. Andrievsky, G.V., Klochkov, V.K., and Derevyanchenko, L.I., Is C60 Fullerene Molecule Toxic?!, Fullerenes, Nanotubes and Carbon Nanostructures, 2005, vol. 13, no. 4, pp. 363–376.

    Article  CAS  Google Scholar 

  4. Kolosnjaj, J., Szwarc, H., and Moussa, F., Toxicity Studies of Fullerenes and Derivatives, Adv. Exp. Med. Biol., 2007, vol. 620, pp. 168–180.

    Article  PubMed  Google Scholar 

  5. Bakry, R. Vallant, R.M., Najam-Ul-Haq, M., Rainer, M., Szabo, Z., Huck, C.W., and Bonn, G.K., Medicinal Applications of Fullerenes, Int. J. Nanomed., 2007, vol. 2, no. 4, pp. 639–649.

    CAS  Google Scholar 

  6. Baati, T., Bourasset, F., Gharbi, N., Njim, L., Abderrabba, M., Kerkeni, A., Szwarc, H., and Moussa, F., The Prolongation of the Lifespan of Rats by Repeated Oral Administration of [60]Fullerene, Biomaterials, 2012, vol. 33, no. 19, pp. 4936–4946.

    Article  PubMed  CAS  Google Scholar 

  7. Quick, K.L., Ali, S.S., Arch, R., Xiong, C., Wozniak, D., and Dugan, L.L., A Carboxyfullerene SOD Mimetic Improves Cognition and Extends the Lifespan of Mice, Neurobiol. Aging, 2008, vol. 29, no. 1, pp. 117–128.

    Article  PubMed  CAS  Google Scholar 

  8. Levi, N., Hantgan, R.R., Lively, M.O., Carroll, D.L., and Prasad, G.L., C60-Fullerenes: Detection of Intracellular Photoluminescence and Lack of Cytotoxic Effects, J. Nanobiotechnol., 2006, vol. 4, no. 14, pp. 14–25.

    Article  Google Scholar 

  9. Mroz, P., Pawlak, A., Satti, M., Lee, H., Wharton, T., Gali, H., Sarna, T., and Hamblin, M.R., Functionalized Fullerenes Mediate Photodynamic Killing of Cancer Cells: Type I Versus Type II Photochemical Mechanism, Free Radic. Biol. Med., 2007, vol. 43, no. 5, pp. 711–719.

    Article  PubMed  CAS  Google Scholar 

  10. Prylutska, S.V., Grynyuk, I.I., Palivoda, K.O., and Matyshevska, O.P., Photoinduced Cytotoxic Effect of Fullerenes C60 on Transformed T-Lymphocytes, Exp. Oncol., 2010, vol. 32, no. 1, pp. 29–32.

    PubMed  CAS  Google Scholar 

  11. Ali, S.S., Hardt, J.I., Quick, K.L., Kim-Han, J.S., Erlanger, B.F., Huang, T.T., Epstein, C.J., and Dugan, L.L., A Biologically Effective Fullerene (C60) Derivative with Superoxide Dismutase Mimetic Properties, Free Radic. Biol. Med., 2004, vol. 37, no. 8, pp. 1191–1202.

    Article  PubMed  CAS  Google Scholar 

  12. Andrievsky, G., Shakhnin, D., Tronza, A., Zhernosekov, D., and Tykhomyrov, A., The Acceleration of Blood Plasma Clot Lysis in the Presence of Hydrated C60 Fullerene Nanostructures in Super-Small Concentration, Fullerenes, Nanotubes and Carbon Nanostructures, 2010, vol. 18, no. 3, pp. 303–311.

    Article  CAS  Google Scholar 

  13. Andrievsky, G.V., Bruskov, V.I., Tykhomyrov, A.A., and Gudkov, S.V., Peculiarities of the Antioxidant and Radioprotective Effects of Hydrated C60 Fullerene Nanostructures in vitro and in vivo, Free Radic. Biol. Med., 2009, vol. 47, no. 6, pp. 786–793.

    Article  PubMed  CAS  Google Scholar 

  14. Yablonskaya, O.I., Voeikov, V.L., Vilenskaya, N.D., Malishenko, S.I., and Novikov, K.N., Effects of Hydrated Fullerenes on the Luminescence of Bacterial Luciferase, of Whole Blood and of Bicarbonate Water Solutions, Luminescence, 2012, vol. 27, no. 2, p. 175.

    Google Scholar 

  15. Chirkova, E.Yu., Golovina, M.E., Nadzharyan, T.L., and Khokhlov, A.N., Cellular Kinetic Model for Studying Geroprotectors and Geropromoters, Dokl. Akad. Nauk SSSR, 1984, vol. 278, no. 6, pp. 1474–1476.

    PubMed  Google Scholar 

  16. Khokhlov, A.N., Cell Proliferation and Aging, Itogi nauki i tekhniki VINITI AN SSSR. Ser. “Obshchie problemy fiziko-khimicheskoi biologii” (Advances in Science and Technology, Series General Problems of Physicochemical Biology), Moscow: VINITI, 1988, vol. 9.

  17. Khokhlov, A.N., Prokhorov, L.Yu., Ivanov, A.S., and Archakov, A.I., Effects of Cholesterol- or 7-Ketocholesterol-Containing Liposomes on Colony-Forming Ability of Cultured Cells, FEBS Lett., 1991, vol. 290, nos. 1–2, pp. 171–172.

    Article  PubMed  CAS  Google Scholar 

  18. Khokhlov, A.N., The Cell Kinetics Model for Determination of Organism Biological Age and for Geroprotectors or Geropromoters Studies, in Biomarkers of Aging: Expression and Regulation. Proceeding, Licastro, F. and Caldarera, C.M., Eds., Bologna: Clueb, 1992, pp. 209–216.

    Google Scholar 

  19. Khokhlov, A.N., Stationary Cell Cultures as a Tool for Gerontological Studies, Ann. N. Y. Acad. Sci., 1992, vol. 663, pp. 475–476.

    Article  PubMed  CAS  Google Scholar 

  20. Khokhlov, A.N., Cytogerontology at the Beginning of the Third Millennium: From “Correlative” to “Gist” Models, Russ. J. Dev. Biol., 2003, vol. 34, no. 5, pp. 321–326.

    Article  Google Scholar 

  21. Khokhlov, A.N., In Search of “Gist” Cytogerontological Models, in Longevity, Aging and Degradation Models in Reliability, Public Health, Medicine and Biology, Antonov, V., Huber, C., Nikulin, M., and Polischook, V., Eds., St. Petersburg: SPbSPU, 2004, vol. 1, pp. 84–92.

    Google Scholar 

  22. Khokhlov, A.N., From Carrel to Hayflick and Back, or What We Got from the 100-Year Cytogerontological Studies, Biophysics, 2010, vol. 55, no. 5, pp. 859–864.

    Article  Google Scholar 

  23. Khokhlov, A.N., Does Aging Need an Own Program or the Existing Development Program Is More Than Enough?, Russ. J. Gen. Chem., 2010, vol. 80, no. 7, pp. 1507–1513.

    Article  CAS  Google Scholar 

  24. Alinkina, E.S., Vorobyova, A.K., Misharina, T.A., Fatkullina, L.D., Burlakova, E.B., and Khokhlov, A.N., Cytogerontological Studies of Biological Activity of Oregano Essential Oil, Mosc. Univ. Biol. Sci. Bull., 2012, vol. 67, no. 2, pp. 52–57.

    Article  Google Scholar 

  25. Andrievsky, G.V., Kosevich, M.V., Vovk, O.M., Shelkovsky, V.S., and Vashchenko, L.A., On the Production of an Aqueous Colloidal Solution of Fullerenes, J. Chem. Soc., Chem. Commun., 1995, no. 12, pp. 1281–1282.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. School of Biology, Moscow State University, Moscow, Russia

    O. I. Yablonskaya, T. S. Ryndina, V. L. Voeikov & A. N. Khokhlov

Authors
  1. O. I. Yablonskaya
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. T. S. Ryndina
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. V. L. Voeikov
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. A. N. Khokhlov
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to A. N. Khokhlov.

Additional information

Original Russian Text © O.I. Yablonskaya, T.S. Ryndina, V.L. Voeikov, A.N. Khokhlov, 2013, published in Vestnik Moskovskogo Universiteta. Biologiya, 2013, No. 1, pp. 14–19.

About this article

Cite this article

Yablonskaya, O.I., Ryndina, T.S., Voeikov, V.L. et al. A paradoxical effect of hydrated C60-fullerene at an ultralow concentration on the viability and aging of cultured Chinese hamster cells. Moscow Univ. Biol.Sci. Bull. 68, 63–68 (2013). https://doi.org/10.3103/S0096392513020107

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.3103/S0096392513020107

Keywords

Search

Navigation