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Effect of petroleum-destructing microorganisms (of the genus Acinetobacter) on the cytogenetic structures of human lymphocytes in cell cultures

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Abstract

The capability of the petroleum-destructing bacteria Acinetobacter calcoaceticus and A. valentis isolated from petroleum to induce karyopathological changes and chromosomal aberrations in human lymphocyte cultures was studied. It was shown that, in contrast to A. valentis, A. calcoaceticus provoked a significant increase in different cytogenetic and chromosomal abnormalities. The majority of the observed chromosomal aberrations (predominantly of the chromatid type) were localized in chromosomes 1 and 2. Meanwhile, abnormalities were found in specific chromosomal regions. Disturbances of the cell division process and changes in the nuclear morphology were observed in the cultures affected by A. calcoaceticus. The most frequent ones were cells displaying micronuclei, nuclear protrusions, lagging of chromosomes and their fragments in mitotic metaphase and anaphase, and multigroup mitoses and C-mitoses. Therefore, in contrast to A. valentis, the petroleum-destructing bacterium A. cacoaceticus has an evident genotoxic effect on human cells in vitro.

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Authors and Affiliations

  1. Siberian State Medical University, Tomsk, Russia

    N. N. Ilyinskikh, E. N. Ilyinskikh & I. N. Ilyinskikh

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  1. N. N. Ilyinskikh
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  2. E. N. Ilyinskikh
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  3. I. N. Ilyinskikh
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Correspondence to N. N. Ilyinskikh.

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Original Russian Text © N.N. Ilyinskikh, E.N. Ilyinskikh, I.N. Ilyinskikh, 2012, published in Tsitologiya i Genetika, 2012, Vol. 46, No. 6, pp. 67–74.

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Ilyinskikh, N.N., Ilyinskikh, E.N. & Ilyinskikh, I.N. Effect of petroleum-destructing microorganisms (of the genus Acinetobacter) on the cytogenetic structures of human lymphocytes in cell cultures. Cytol. Genet. 46, 384–389 (2012). https://doi.org/10.3103/S0095452712060047

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  • DOI: https://doi.org/10.3103/S0095452712060047

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