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Polarized fluorescence in investigation of rotational diffusion of the fluorescein family markers in bovine serum albumin solutions

  • Biophysics and Medical Physics
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Moscow University Physics Bulletin Aims and scope

Abstract

The analysis of polarized fluorescence of the fluorescein family markers was conducted and parameters of their rotational diffusion in bovine serum albumin solutions (BSA) were determined. The degree of fluorescence anisotropy of the markers increases in the BSA solutions, as well as the time of rotational relaxation of the markers, while the rotational-diffusion coefficient of the markers decreases. The differences in the rotational-diffusion parameters between the markers are determined by the values of the electronegativity of the atoms in their structural formulas: the increase of the electronegativity of the atoms in the structural formulas of the markers results in the increase of the degree of fluorescence anisotropy, a decrease of the rotational-diffusion coefficient, and in the increase of the rotational-relaxation time both in the solutions without the protein and with BSA.

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Authors and Affiliations

  1. Department of Physics, Moscow State University, Moscow, Russia

    I. M. Vlasova, A. A. Kuleshova, A. A. Vlasov & A. M. Saletskii

Authors
  1. I. M. Vlasova
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  2. A. A. Kuleshova
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  3. A. A. Vlasov
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  4. A. M. Saletskii
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Corresponding author

Correspondence to I. M. Vlasova.

Additional information

Original Russian Text © I.M. Vlasova, A.A. Kuleshova, A.A. Vlasov, A.M. Saletskii, 2014, published in Vestnik Moskovskogo Universiteta. Fizika, 2014, No. 5, pp. 36–40.

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Vlasova, I.M., Kuleshova, A.A., Vlasov, A.A. et al. Polarized fluorescence in investigation of rotational diffusion of the fluorescein family markers in bovine serum albumin solutions. Moscow Univ. Phys. 69, 401–405 (2014). https://doi.org/10.3103/S0027134914050099

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  • DOI: https://doi.org/10.3103/S0027134914050099

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