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Signal enhancement from fluorescently labeled exosomes: Theoretical analysis of fluorescence in the presence of plasmonic nanoparticles

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Moscow University Chemistry Bulletin Aims and scope

Abstract

A method for measuring the concentration of exosomes in biological objects using selective fluorescence labeling followed by nonselective enhancement by plasmonic nanoparticles is suggested. Theoretical investigation of the suggested approach is conducted. A model for description of the interaction of the incident wave, fluorophore, and plasmonic nanoparticles is developed. Calculations of the fluorescence enhancement of the molecule in the vicinity of the gold or silver nanoparticles are conducted with consideration of the actual dispersion of dielectric permittivity of metals. The physical mechanisms of the fluorescence enhancement—change of the local field in the vicinity of nanoparticles, difference in the radiation efficiency of the fluorophore and nanoparticle, and superradiance—are analyzed.

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Authors and Affiliations

  1. Dukhov All-Russia Research Institute of Automatics, Moscow, Russia

    I. A. Nechepurenko, A. V. Dorofeenko & A. P. Vinogradov

  2. Moscow Institute of Physics and Technology (State University), Dolgoprudnyi, Moscow oblast, Russia

    I. A. Nechepurenko, A. V. Dorofeenko & A. P. Vinogradov

  3. Institute of Theoretical and Applied Electrodynamics, Russian Academy of Sciences, Moscow, Russia

    A. V. Dorofeenko & A. P. Vinogradov

  4. Department of Chemistry, Moscow State University, Moscow, Russia

    E. G. Evtushenko & I. N. Kurochkin

Authors
  1. I. A. Nechepurenko
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  2. A. V. Dorofeenko
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  3. A. P. Vinogradov
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  4. E. G. Evtushenko
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  5. I. N. Kurochkin
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Corresponding author

Correspondence to A. V. Dorofeenko.

Additional information

Original Russian Text © I.A. Nechepurenko, A.V. Dorofeenko, A.P. Vinogradov, E.G. Evtushenko, I.N. Kurochkin, 2015, published in Vestnik Moskovskogo Universiteta. Khimiya, 2015, No. 3, pp. 132–141.

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Nechepurenko, I.A., Dorofeenko, A.V., Vinogradov, A.P. et al. Signal enhancement from fluorescently labeled exosomes: Theoretical analysis of fluorescence in the presence of plasmonic nanoparticles. Moscow Univ. Chem. Bull. 70, 108–116 (2015). https://doi.org/10.3103/S0027131415030074

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  • DOI: https://doi.org/10.3103/S0027131415030074

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