Cytology and Genetics

, Volume 44, Issue 1, pp 19–22

Formation of multicellular aggregates under different conditions of microenvironment

  • L. V. Garmanchuk
  • E. M. Perepelitsyna
  • M. V. Sydorenko
  • L. I. Ostapchenko
Article

DOI: 10.3103/S0095452710010044

Cite this article as:
Garmanchuk, L.V., Perepelitsyna, E.M., Sydorenko, M.V. et al. Cytol. Genet. (2010) 44: 19. doi:10.3103/S0095452710010044

Abstract

Multicellular aggregates (spheroids) represent an intermittent level between monolayer growing cells and tissue culture. Spheroids are rather objective model of the three-dimensional growth and organization, the cell-to-cell interactions and influence of microenvironmental conditions on tumour microaggre-gates. In our work formation and growth of spheroids depends on concentration of CMC and FCS. Conditions of microenvironment influence on intensiveness of proliferation as well as on cells adhesiveness and formation of microaggregates.

Copyright information

© Allerton Press, Inc. 2010

Authors and Affiliations

  • L. V. Garmanchuk
    • 1
  • E. M. Perepelitsyna
    • 1
  • M. V. Sydorenko
    • 1
  • L. I. Ostapchenko
    • 2
  1. 1.Department of Biotecftntcal Problems of Diagnostics Institute Problems of Cryobiology and Criomedicine NAN UkraineKievUkraine
  2. 2.Taras Shevchenko Kiev National UniversityKievUkraine

Personalised recommendations