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Ploidy analysis of Cymbidium, Phalaenopsis, Dendrobium and Paphiopedillum (Orchidaceae), and Spathiphyllum and Syngonium (Araceae)

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Abstract

Endopolyploidy is typical and wide-spread in the Orchidaceae. In this study, an attempt was made to quantify the ploidy of mature callus and mature protocorm-like bodies (PLBs) of nine hybrid Cymbidium cultivars cultured in vitro, as well as different flower parts (dorsal sepal, petal, lateral sepal, labellum, pedicel, column, anther cap, stigmatic surface) and youngest leaf of Phalaenopsis and Paphiopedillum and in leaf tissues of in vitro Spathiphyllum and Syngonium plants. Polyploidy was detected in in vitro cultures, primarily in PLBs, ant to a limited extent in floral tissue: 8C was detected in the pedicel and column, but never exceeding 3%. This study expands on the number and breadth of cases of endopolyploidy in orchids, with novel information about Paphiopedillum. These findings may be useful for better understanding developmental processes in vitro or in planta, and may be the first step to revealing evolutionary or adaptive mechanisms of survival of orchids in response to environmental or abiotic stresses.

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Abbreviations

PGR:

plant growth regulator

PLB:

protocorm-like body

TC medium:

Teixeira Cymbidium medium

TCL:

thin cell layer

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Authors and Affiliations

  1. Faculty of Agriculture and Graduate School of Agriculture, Kagawa University, Miki-cho, Kagawa, 761-0795, Japan

    Jaime A. Teixeira da Silva, Dam T. T. Giang & Michio Tanaka

  2. Research Institute of Nyíregyháza, Research Institutes and Study Farm, Centre for Agricultural and Applied Economic Sciences, University of Debrecen, Nyíregyháza, P.O. Box 12, H-4400, Hungary

    Judit Dobránszki

  3. Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, 510650, China

    Songjun Zeng

  4. Miki-cho post office, P.O. Box 7, Ikenobe 3011-2, Kagawa-ken, 761-0799, Japan

    Jaime A. Teixeira da Silva

Authors
  1. Jaime A. Teixeira da Silva
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  2. Dam T. T. Giang
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  3. Judit Dobránszki
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  4. Songjun Zeng
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  5. Michio Tanaka
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Correspondence to Jaime A. Teixeira da Silva.

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Teixeira da Silva, J.A., Giang, D.T.T., Dobránszki, J. et al. Ploidy analysis of Cymbidium, Phalaenopsis, Dendrobium and Paphiopedillum (Orchidaceae), and Spathiphyllum and Syngonium (Araceae). Biologia 69, 750–755 (2014). https://doi.org/10.2478/s11756-014-0370-z

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  • DOI: https://doi.org/10.2478/s11756-014-0370-z

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