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Purification and characterization of an exo-polygalacturonase secreted by Rhizopus oryzae MTCC 1987 and its role in retting of Crotalaria juncea fibre

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Abstract

An acidic polygalacturonase (PG) secreted by Rhizopus oryzae MTCC-1987 in submerged fermentation condition has been purified to electrophoretic homogeneity using ammonium sulphate fractionation and anion exchange chromatography on diethylaminoethyl cellulose. The purified enzyme gave a single protein band in sodium dodecyl sulphatepolyacrylamide gel electrophoresis analysis with a molecular mass corresponding to 75.5 kDa. The K m and k cat values of the PG were 2.7 mg/mL and 2.23 × 103 s−1, respectively, using citrus polygalacturonic acid as the substrate. The optimum pH of the purified PG was 5.0 and it does not loose activity appreciably if left for 24 hours in the pH range from 5.0 to 12.0. The optimum temperature of purified enzyme was 50°C and the enzyme does not loose activity below 30°C if exposed for two hours. The purified enzyme showed complete inhibition with 1 mM Ag+, Hg2+ and KMnO4, while it was stimulated to some extent by Co2+. The purified PG exhibited retting of Crotalaria juncea fibre in absence of ethylenediaminetetraacetic acid.

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Abbreviations

DEAE:

diethylaminoethyl

DNSA:

3,5-dinitrosalicylic acid

EDTA:

ethylenediaminetetraacetic acid

PG:

polygalacturonase

PGA:

polygalacturonic acid

SDS-PAGE:

sodium dodecyl sulphate polyacrylamide gel electrophoresis

TLC:

thin layer chromatography

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Authors and Affiliations

  1. Department of Biotechnology, D.D.U. Gorakhpur University, Gorakhpur, (U.P.), 273009, India

    Sangeeta Yadav, Gautam Anand, Amit K. Dubey & Dinesh Yadav

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  1. Sangeeta Yadav
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  2. Gautam Anand
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  3. Amit K. Dubey
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  4. Dinesh Yadav
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Correspondence to Sangeeta Yadav.

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Yadav, S., Anand, G., Dubey, A.K. et al. Purification and characterization of an exo-polygalacturonase secreted by Rhizopus oryzae MTCC 1987 and its role in retting of Crotalaria juncea fibre. Biologia 67, 1069–1074 (2012). https://doi.org/10.2478/s11756-012-0122-x

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  • DOI: https://doi.org/10.2478/s11756-012-0122-x

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